Detailed information of TA system TA078458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2598791..2599442
• Replicon: chromosome
• Accession: NZ_CP022109
• Organism: Lacticaseibacillus rhamnosus strain JCM1553

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: CEA83_RS13045
• Protein ID: WP_005686631.1
• Coordinates: 2598791..2599174 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: CEA83_RS13050
• Protein ID: WP_005686632.1
• Coordinates: 2599194..2599442 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA83_RS13030	2597750..2598277	-	528	WP_005691221.1	QueT transporter family protein	-
CEA83_RS13045	2598791..2599174	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEA83_RS13050	2599194..2599442	-	249	WP_005686632.1	antitoxin	Antitoxin
CEA83_RS13055	2599554..2600693	-	1140	WP_005691215.1	alanine racemase	-
CEA83_RS13060	2600680..2601054	-	375	WP_005686634.1	holo-ACP synthase	-
CEA83_RS13065	2601223..2602731	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
CEA83_RS13070	2603005..2604393	-	1389	WP_014571607.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T78458 WP_005686631.1 NZ_CP022109:c2599174-2598791 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T78458 NZ_CP022109:c2599174-2598791 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT78458 WP_005686632.1 NZ_CP022109:c2599442-2599194 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT78458 NZ_CP022109:c2599442-2599194 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19