Detailed information of TA system TA078457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2501883..2502445
• Replicon: chromosome
• Accession: NZ_CP022109
• Organism: Lacticaseibacillus rhamnosus strain JCM1553

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C2JYM4
• Locus tag: CEA83_RS12540
• Protein ID: WP_005692155.1
• Coordinates: 2501883..2502230 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CEA83_RS12545
• Protein ID: WP_014571589.1
• Coordinates: 2502230..2502445 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA83_RS12510	2497378..2498619	-	1242	WP_005692160.1	acyltransferase	-
CEA83_RS12515	2498616..2499317	-	702	WP_005692159.1	ABC transporter ATP-binding protein	-
CEA83_RS12520	2499310..2500128	-	819	WP_014571588.1	ABC transporter permease subunit	-
CEA83_RS12525	2500369..2501037	-	669	WP_005692157.1	tRNA ligase	-
CEA83_RS12530	2501217..2501360	+	144	WP_015764720.1	hypothetical protein	-
CEA83_RS12540	2501883..2502230	-	348	WP_005692155.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEA83_RS12545	2502230..2502445	-	216	WP_014571589.1	hypothetical protein	Antitoxin
CEA83_RS12550	2502542..2504377	-	1836	WP_015764721.1	ABC transporter ATP-binding protein/permease	-
CEA83_RS12555	2504364..2506154	-	1791	WP_015764722.1	ABC transporter ATP-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13150.01 Da
• Isoelectric Point: 7.9817

>T78457 WP_005692155.1 NZ_CP022109:c2502230-2501883 [Lacticaseibacillus rhamnosus]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF

Nucleotide

• Length: 348 bp

>T78457 NZ_CP022109:c2502230-2501883 [Lacticaseibacillus rhamnosus]
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7986.13 Da
• Isoelectric Point: 5.7995

>AT78457 WP_014571589.1 NZ_CP022109:c2502445-2502230 [Lacticaseibacillus rhamnosus]
MDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE

Nucleotide

• Length: 216 bp

>AT78457 NZ_CP022109:c2502445-2502230 [Lacticaseibacillus rhamnosus]
ATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGAGGC
GTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAACCAT
TATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA

Structures

Toxin

• AlphaFold DB: A0A7Z9DS75