Detailed information of TA system TA078455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 397323..397960
• Replicon: chromosome
• Accession: NZ_CP022106
• Organism: Halobacillus halophilus strain HL2HP6

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: I0JI00
• Locus tag: CEH05_RS02060
• Protein ID: WP_014641675.1
• Coordinates: 397610..397960 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: I0JHZ9
• Locus tag: CEH05_RS02055
• Protein ID: WP_014641674.1
• Coordinates: 397323..397604 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEH05_RS02035	392859..393215	+	357	WP_014641670.1	holo-ACP synthase	-
CEH05_RS02040	393287..394816	+	1530	WP_014641671.1	NAD(P)H-hydrate dehydratase	-
CEH05_RS02045	394859..395878	+	1020	WP_014641672.1	outer membrane lipoprotein carrier protein LolA	-
CEH05_RS02050	396008..397159	+	1152	WP_041601510.1	alanine racemase	-
CEH05_RS02055	397323..397604	+	282	WP_014641674.1	hypothetical protein	Antitoxin
CEH05_RS02060	397610..397960	+	351	WP_014641675.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEH05_RS02065	398219..399088	+	870	WP_014641676.1	RsbT co-antagonist protein RsbRA	-
CEH05_RS02070	399085..399450	+	366	WP_173380425.1	STAS domain-containing protein	-
CEH05_RS02075	399454..399855	+	402	WP_014641678.1	anti-sigma regulatory factor	-
CEH05_RS02080	399870..400880	+	1011	WP_014641679.1	PP2C family protein-serine/threonine phosphatase	-
CEH05_RS02085	400946..401278	+	333	WP_014641680.1	STAS domain-containing protein	-
CEH05_RS02090	401278..401754	+	477	WP_014641681.1	anti-sigma B factor RsbW	-
CEH05_RS02095	401726..402505	+	780	WP_014641682.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12874.97 Da
• Isoelectric Point: 5.8431

>T78455 WP_014641675.1 NZ_CP022106:397610-397960 [Halobacillus halophilus]
VIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAEKYGFERNSVILLEQI
RTIDKQRLTDKITQLDDAMMLEVNKALQISLGLIDF

Nucleotide

• Length: 351 bp

>T78455 NZ_CP022106:397610-397960 [Halobacillus halophilus]
GTGATAGTCAAACGAGGAGACGTTTATTTCGCTGATTTGTCCCCGGTAGTGGGATCAGAGCAAGGCGGGGTTCGTCCTGT
ACTCATCCTTCAAAATGATATCGGTAATCGTTTTAGCCCTACAGTTATCGTAGCTGCAATTACAGCGCAAATACAAAAAG
CCAAACTTCCCACACACGTAGAAATCAATGCAGAAAAATATGGGTTCGAACGAAATTCTGTGATCCTATTGGAACAAATC
AGAACGATTGATAAACAGCGTTTGACCGACAAGATCACTCAACTGGACGACGCAATGATGCTGGAAGTCAACAAAGCGTT
GCAGATCAGCCTTGGATTAATTGATTTCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10867.42 Da
• Isoelectric Point: 4.5686

>AT78455 WP_014641674.1 NZ_CP022106:397323-397604 [Halobacillus halophilus]
MVLSDSMQEIVIRIPDNLLNEVDGFIQLENSDRSDFMCQATKMYLREKKQRHVRESMRRGYMEMAKINLNIASEMFQAEE
EAEHTLEQLVGGV

Nucleotide

• Length: 282 bp

>AT78455 NZ_CP022106:397323-397604 [Halobacillus halophilus]
ATGGTTTTGTCCGATAGCATGCAGGAGATTGTCATCCGGATTCCGGATAACCTACTCAATGAAGTGGACGGCTTTATTCA
ACTCGAAAATAGTGATCGGAGCGATTTCATGTGTCAAGCAACTAAAATGTATTTGCGTGAGAAGAAACAGCGTCATGTCA
GGGAATCCATGCGAAGAGGCTACATGGAAATGGCGAAAATTAATTTAAACATCGCTTCAGAAATGTTTCAAGCTGAAGAG
GAGGCAGAACACACCCTGGAGCAATTAGTGGGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: I0JI00

Antitoxin

• AlphaFold DB: I0JHZ9