Detailed information of TA system TA078434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 522472..522988
• Replicon: chromosome
• Accession: NZ_CP022100
• Organism: Vibrio anguillarum strain S3 4/9

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A244CLX5
• Locus tag: CEG15_RS16080
• Protein ID: WP_069509627.1
• Coordinates: 522719..522988 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: CEG15_RS16075
• Protein ID: WP_088729558.1
• Coordinates: 522472..522726 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEG15_RS16045	518029..518358	+	330	WP_047691665.1	hypothetical protein	-
CEG15_RS16050	518518..518862	+	345	WP_088729555.1	hypothetical protein	-
CEG15_RS16065	520458..521099	+	642	WP_088729556.1	hypothetical protein	-
CEG15_RS16070	521183..521704	+	522	WP_088729557.1	GNAT family N-acetyltransferase	-
CEG15_RS16075	522472..522726	+	255	WP_088729558.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CEG15_RS16080	522719..522988	+	270	WP_069509627.1	Txe/YoeB family addiction module toxin	Toxin
CEG15_RS16085	523177..523797	+	621	WP_088729559.1	hypothetical protein	-
CEG15_RS16090	523947..524720	+	774	WP_088729560.1	hypothetical protein	-
CEG15_RS16095	524803..524943	+	141	WP_088729561.1	DUF3265 domain-containing protein	-
CEG15_RS16100	524951..525250	-	300	WP_019282621.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CEG15_RS16105	525247..525489	-	243	WP_072609711.1	type II toxin-antitoxin system ParD family antitoxin	-
CEG15_RS16110	525573..525665	+	93	WP_193784381.1	DUF3265 domain-containing protein	-
CEG15_RS16115	525704..526210	+	507	WP_157724208.1	hypothetical protein	-
CEG15_RS16120	526324..526788	+	465	WP_064626859.1	ASCH domain-containing protein	-
CEG15_RS16125	526781..526903	+	123	WP_081265636.1	DUF3265 domain-containing protein	-
CEG15_RS16130	526946..527584	+	639	WP_017045405.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	523947..546303	22356
	-	inside	Integron	-	-	435402..647524	212122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10570.95 Da
• Isoelectric Point: 8.8578

>T78434 WP_069509627.1 NZ_CP022100:522719-522988 [Vibrio anguillarum]
MSSSQRLLSWTDEAWGDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T78434 NZ_CP022100:522719-522988 [Vibrio anguillarum]
ATGAGTAGTAGTCAACGTTTACTATCGTGGACTGATGAGGCTTGGGGTGATTATCTGTATTGGCAAACCCAAGATAAGAA
AACACTTAAGCGTATCAATAAACTCATCAATGATGTTAAGCGTTCTCCTTTTGAGGGTATTGGTAAACCCGAGCCGTTAA
AAGAGAACTTATCTGGCTTTTGGTCTCGCCGCATTGACGATACTAATAGACTTGTTTACGCAGTCGATGATCAAGCGATA
ACAATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9453.56 Da
• Isoelectric Point: 4.7483

>AT78434 WP_088729558.1 NZ_CP022100:522472-522726 [Vibrio anguillarum]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNAEHLNRSIAQYRAGQTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT78434 NZ_CP022100:522472-522726 [Vibrio anguillarum]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTAGACGGTGTAGTTAATGACGCAGACACAAC
AGTCATTACACGTCGTGACTCTGAAGATGCTGTGGTTATGTCTTTAGACTACTACAACAGTCTTATGGAGACAGTGCACC
TACTACGCTCTCCTCAAAATGCTGAACACCTAAACAGATCAATAGCACAGTACCGTGCTGGTCAAACAACCGCACGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: A0A244CLX5