Detailed information of TA system TA078419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2540242..2540806
• Replicon: chromosome
• Accession: NZ_CP022095
• Organism: Mycobacterium avium subsp. paratuberculosis strain FDAARGOS_305

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: CEP84_RS12600
• Protein ID: WP_003875391.1
• Coordinates: 2540435..2540806 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: CEP84_RS12595
• Protein ID: WP_003875390.1
• Coordinates: 2540242..2540448 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP84_RS12575	2535495..2536493	+	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CEP84_RS12580	2536517..2537698	-	1182	WP_003875387.1	hypothetical protein	-
CEP84_RS12585	2537856..2538929	+	1074	WP_010949695.1	redox-regulated ATPase YchF	-
CEP84_RS12590	2539129..2540073	+	945	WP_003875389.1	HNH endonuclease	-
CEP84_RS12595	2540242..2540448	+	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CEP84_RS12600	2540435..2540806	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
CEP84_RS12605	2540906..2541346	+	441	WP_010949694.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
CEP84_RS12610	2541402..2541677	+	276	WP_003878531.1	hypothetical protein	-
CEP84_RS12615	2541779..2542177	+	399	WP_003875394.1	VOC family protein	-
CEP84_RS12620	2542244..2542627	+	384	WP_003875395.1	hypothetical protein	-
CEP84_RS12625	2542697..2543050	+	354	WP_003875396.1	hypothetical protein	-
CEP84_RS12630	2543228..2543731	+	504	WP_010949693.1	glycoside hydrolase	-
CEP84_RS12635	2544142..2544466	+	325	Protein_2469	antibiotic biosynthesis monooxygenase	-
CEP84_RS12640	2544471..2545154	-	684	WP_003875399.1	hypothetical protein	-
CEP84_RS12645	2545272..2545469	+	198	WP_016706054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T78419 WP_003875391.1 NZ_CP022095:2540435-2540806 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T78419 NZ_CP022095:2540435-2540806 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT78419 WP_003875390.1 NZ_CP022095:2540242-2540448 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT78419 NZ_CP022095:2540242-2540448 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2