Detailed information of TA system TA078417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2397505..2398034
• Replicon: chromosome
• Accession: NZ_CP022093
• Organism: Staphylococcus saprophyticus strain FDAARGOS_355

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q49Z22
• Locus tag: CEQ33_RS12160
• Protein ID: WP_011302703.1
• Coordinates: 2397672..2398034 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q49Z23
• Locus tag: CEQ33_RS12155
• Protein ID: WP_011302702.1
• Coordinates: 2397505..2397675 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ33_RS12130	2393236..2393715	+	480	WP_011302698.1	PH domain-containing protein	-
CEQ33_RS12135	2393708..2395246	+	1539	WP_041784781.1	PH domain-containing protein	-
CEQ33_RS12140	2395239..2395739	+	501	WP_011302700.1	PH domain-containing protein	-
CEQ33_RS12145	2395803..2396153	+	351	WP_002482750.1	holo-ACP synthase	-
CEQ33_RS12150	2396269..2397417	+	1149	WP_011302701.1	alanine racemase	-
CEQ33_RS12155	2397505..2397675	+	171	WP_011302702.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CEQ33_RS12160	2397672..2398034	+	363	WP_011302703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEQ33_RS12165	2398371..2399372	+	1002	WP_011302704.1	PP2C family protein-serine/threonine phosphatase	-
CEQ33_RS12170	2399451..2399777	+	327	WP_011302705.1	anti-sigma factor antagonist	-
CEQ33_RS12175	2399779..2400261	+	483	WP_002482755.1	anti-sigma B factor RsbW	-
CEQ33_RS12180	2400236..2401001	+	766	Protein_2269	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13482.70 Da
• Isoelectric Point: 10.3173

>T78417 WP_011302703.1 NZ_CP022093:2397672-2398034 [Staphylococcus saprophyticus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSDKKMKEVNNAIDISLGLHTIRSHKA

Nucleotide

• Length: 363 bp

>T78417 NZ_CP022093:2397672-2398034 [Staphylococcus saprophyticus]
ATGATGAGACGTGGTGACGTTTATTTAGCTGATTTATCACCAGTCCAGGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
AATTATACAAAACGATACTGGTAATAAATATAGTCCTACCGTAATTGTAGCCGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAATTAGATAAAGATTCTGTTATATTGCTAGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACTTATCTTTCTGATAAAAAAATGAAAGAAGTTAATAATGCCATTGA
TATTAGTTTAGGATTACATACTATTAGGAGTCACAAAGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6332.00 Da
• Isoelectric Point: 4.0121

>AT78417 WP_011302702.1 NZ_CP022093:2397505-2397675 [Staphylococcus saprophyticus]
MASFNQSRIHNIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT78417 NZ_CP022093:2397505-2397675 [Staphylococcus saprophyticus]
ATGGCAAGTTTCAACCAAAGTAGAATTCATAATATAGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGATTTGAA
CCTCTCCCTCGCAGCAGAAGCATTTCCGTTTGAATGTGAAGCTTGTGATTGCAACGAAGTATATATTAGCTCCAAGAATA
ATAATGAATGA

Structures

Toxin

• AlphaFold DB: Q49Z22

Antitoxin

• AlphaFold DB: Q49Z23