Detailed information of TA system TA078402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 144253..144845
• Replicon: chromosome
• Accession: NZ_CP022089
• Organism: Shewanella sp. FDAARGOS_354

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CEQ32_RS00755
• Protein ID: WP_088585326.1
• Coordinates: 144591..144845 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A252ENK1
• Locus tag: CEQ32_RS00750
• Protein ID: WP_014610929.1
• Coordinates: 144253..144594 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ32_RS00725	139870..140274	+	405	WP_069453179.1	IS200/IS605 family transposase	-
CEQ32_RS00730	140381..140653	+	273	WP_069453183.1	CcdB family protein	-
CEQ32_RS00735	141008..142375	+	1368	WP_014610930.1	HipA domain-containing protein	-
CEQ32_RS00740	142372..142752	+	381	WP_086904820.1	helix-turn-helix domain-containing protein	-
CEQ32_RS00745	142887..144095	+	1209	WP_001206290.1	IS4-like element IS10A family transposase	-
CEQ32_RS00750	144253..144594	-	342	WP_014610929.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CEQ32_RS00755	144591..144845	-	255	WP_088585326.1	type II toxin-antitoxin system HicA family toxin	Toxin
CEQ32_RS00760	145078..145977	+	900	WP_088585327.1	IS982 family transposase	-
CEQ32_RS00765	146074..147223	+	1150	Protein_147	IS3 family transposase	-
CEQ32_RS00770	147254..147805	+	552	Protein_148	GDP-mannose 4,6-dehydratase	-
CEQ32_RS00775	147904..148053	+	150	WP_088586927.1	tyrosine-type recombinase/integrase	-
CEQ32_RS22900	148131..148295	-	165	WP_014610884.1	hypothetical protein	-
CEQ32_RS00780	148317..149042	-	726	WP_088585328.1	zeta toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	142887..146995	4108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (55-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9525.05 Da
• Isoelectric Point: 10.1453

>T78402 WP_088585326.1 NZ_CP022089:c144845-144591 [Shewanella sp. FDAARGOS_354]
MNKKHLRTLTAIFARPISGTIKWSDIEALFIALGADIEEREGSRIGVVLFGEVQVYHRPHPQKETDKDAVISIKKWLERN
GVKA

Nucleotide

• Length: 255 bp

>T78402 NZ_CP022089:c144845-144591 [Shewanella sp. FDAARGOS_354]
ATGAACAAAAAACATTTAAGAACGTTGACGGCTATCTTTGCTAGGCCTATCAGCGGAACTATCAAGTGGTCTGATATTGA
AGCGCTGTTTATTGCTCTAGGGGCTGACATTGAAGAGCGTGAGGGGTCTCGAATTGGTGTGGTGCTGTTTGGTGAAGTTC
AGGTATATCACCGCCCACATCCTCAGAAAGAAACTGACAAAGACGCAGTGATAAGCATCAAAAAATGGTTAGAAAGGAAC
GGGGTGAAAGCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12379.91 Da
• Isoelectric Point: 4.3992

>AT78402 WP_014610929.1 NZ_CP022089:c144594-144253 [Shewanella sp. FDAARGOS_354]
MKNLMMINGVKAFIDYDPDAETFRGEFVGLNGGADFYGESVAQLEAEGAKSLSVFLEMCQERGIEPYKNFSGKFNVRISP
EVHARLNEIALSQSISLNAAVENAVNDYIAHSA

Nucleotide

• Length: 342 bp

>AT78402 NZ_CP022089:c144594-144253 [Shewanella sp. FDAARGOS_354]
ATGAAAAATCTTATGATGATCAACGGGGTAAAAGCCTTTATTGATTATGATCCAGACGCTGAAACATTCCGTGGTGAGTT
CGTAGGCTTAAACGGCGGCGCGGATTTCTATGGTGAAAGCGTCGCTCAATTGGAAGCCGAGGGCGCGAAGTCATTAAGCG
TTTTTTTAGAGATGTGTCAGGAGCGTGGCATTGAGCCTTACAAAAACTTCTCTGGCAAATTCAACGTTCGGATCTCGCCA
GAGGTTCACGCTCGACTTAATGAAATTGCGCTATCGCAAAGCATTAGTTTGAATGCAGCGGTAGAAAATGCAGTCAATGA
CTACATTGCACATTCAGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A252ENK1