Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59816..60471 Replicon plasmid unnamed2
Accession NZ_CP022087
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name vagD Uniprot ID A0A2H3M3N3
Locus tag CEQ26_RS28775 Protein ID WP_001392130.1
Coordinates 60070..60471 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A0E0YAG4
Locus tag CEQ26_RS28770 Protein ID WP_001261288.1
Coordinates 59816..60046 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS28735 54868..55284 - 417 WP_001278815.1 plasmid partitioning/stability family protein -
CEQ26_RS28740 55277..56257 - 981 WP_000688504.1 plasmid segregation protein ParM -
CEQ26_RS28745 56671..56979 - 309 WP_000030204.1 molecular chaperone GroEL -
CEQ26_RS28750 57066..57710 - 645 WP_001144032.1 ParA family protein -
CEQ26_RS28755 57889..58695 - 807 WP_000016961.1 site-specific integrase -
CEQ26_RS28760 58696..59001 - 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB -
CEQ26_RS28765 59003..59221 - 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA -
CEQ26_RS28770 59816..60046 + 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CEQ26_RS28775 60070..60471 + 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin Toxin
CEQ26_RS28780 60554..60703 - 150 Protein_70 transposase domain-containing protein -
CEQ26_RS28790 61795..62772 + 978 WP_001387465.1 RepB family plasmid replication initiator protein -
CEQ26_RS28795 63051..63791 - 741 WP_001066951.1 tyrosine-type recombinase/integrase -
CEQ26_RS28800 63912..64037 - 126 WP_032159539.1 hypothetical protein -
CEQ26_RS28805 64160..64321 - 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
CEQ26_RS28810 64437..64768 - 332 Protein_75 type II toxin-antitoxin system ParD family antitoxin -
CEQ26_RS28815 64774..65049 - 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin -
CEQ26_RS28820 65049..65333 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggA / aggB / aggC / aggD 1..75559 75559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14647.00 Da        Isoelectric Point: 6.7123

>T78396 WP_001392130.1 NZ_CP022087:60070-60471 [Escherichia coli O104:H4]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 402 bp

>T78396 NZ_CP022087:60070-60471 [Escherichia coli O104:H4]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8684.76 Da        Isoelectric Point: 4.3654

>AT78396 WP_001261288.1 NZ_CP022087:59816-60046 [Escherichia coli O104:H4]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Download         Length: 231 bp

>AT78396 NZ_CP022087:59816-60046 [Escherichia coli O104:H4]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H3M3N3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0YAG4

References