Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4454337..4455138 Replicon chromosome
Accession NZ_CP022086
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag CEQ26_RS24230 Protein ID WP_001094430.1
Coordinates 4454337..4454714 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag CEQ26_RS24235 Protein ID WP_001285620.1
Coordinates 4454761..4455138 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS24205 4450421..4451959 - 1539 WP_001187182.1 lysine decarboxylation/transport transcriptional activator CadC -
CEQ26_RS24215 4452708..4453550 - 843 WP_001290187.1 DUF4942 domain-containing protein -
CEQ26_RS24220 4453635..4453832 - 198 WP_000772029.1 DUF957 domain-containing protein -
CEQ26_RS24225 4453852..4454340 - 489 WP_000761715.1 hypothetical protein -
CEQ26_RS24230 4454337..4454714 - 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
CEQ26_RS24235 4454761..4455138 - 378 WP_001285620.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
CEQ26_RS24240 4455218..4455439 - 222 WP_000692350.1 DUF987 domain-containing protein -
CEQ26_RS24245 4455526..4456001 - 476 Protein_4259 RadC family protein -
CEQ26_RS24250 4456017..4456490 - 474 WP_000855057.1 antirestriction protein -
CEQ26_RS29535 4456832..4456957 - 126 Protein_4261 DUF945 domain-containing protein -
CEQ26_RS24265 4457014..4457753 + 740 Protein_4262 IS3 family transposase -
CEQ26_RS24275 4458426..4459934 + 1509 WP_001189118.1 group II intron reverse transcriptase/maturase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T78392 WP_001094430.1 NZ_CP022086:c4454714-4454337 [Escherichia coli O104:H4]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T78392 NZ_CP022086:c4454714-4454337 [Escherichia coli O104:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT78392 WP_001285620.1 NZ_CP022086:c4455138-4454761 [Escherichia coli O104:H4]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT78392 NZ_CP022086:c4455138-4454761 [Escherichia coli O104:H4]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References