Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4442608..4443105 Replicon chromosome
Accession NZ_CP022086
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CEQ26_RS24175 Protein ID WP_001173343.1
Coordinates 4442932..4443105 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CEQ26_RS24170 Protein ID WP_000398619.1
Coordinates 4442608..4442904 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS24145 4439339..4440058 - 720 WP_000611288.1 two-component system response regulator DcuR -
CEQ26_RS24150 4440055..4441686 - 1632 WP_001216486.1 sensor histidine kinase -
CEQ26_RS24155 4441867..4442097 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
CEQ26_RS24160 4442109..4442381 + 273 WP_000405647.1 N-acetyltransferase -
CEQ26_RS24170 4442608..4442904 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CEQ26_RS24175 4442932..4443105 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CEQ26_RS24180 4443224..4444741 - 1518 WP_001295074.1 lysine--tRNA ligase -
CEQ26_RS24185 4444978..4446435 - 1458 WP_000856837.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T78391 WP_001173343.1 NZ_CP022086:4442932-4443105 [Escherichia coli O104:H4]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T78391 NZ_CP022086:4442932-4443105 [Escherichia coli O104:H4]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT78391 WP_000398619.1 NZ_CP022086:4442608-4442904 [Escherichia coli O104:H4]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT78391 NZ_CP022086:4442608-4442904 [Escherichia coli O104:H4]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References