Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2941156..2941810 Replicon chromosome
Accession NZ_CP022086
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag CEQ26_RS16265 Protein ID WP_000244772.1
Coordinates 2941156..2941563 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CEQ26_RS16270 Protein ID WP_000354046.1
Coordinates 2941544..2941810 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS16245 2937113..2938846 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
CEQ26_RS16250 2938852..2939562 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CEQ26_RS16255 2939587..2940483 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CEQ26_RS16260 2940595..2941116 + 522 WP_001055874.1 flavodoxin FldB -
CEQ26_RS16265 2941156..2941563 - 408 WP_000244772.1 protein YgfX Toxin
CEQ26_RS16270 2941544..2941810 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CEQ26_RS16275 2942053..2943033 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CEQ26_RS16280 2943111..2943770 - 660 WP_000250274.1 hemolysin III family protein -
CEQ26_RS16285 2943934..2944245 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CEQ26_RS16290 2944290..2945723 + 1434 WP_001387034.1 6-phospho-beta-glucosidase BglA -
CEQ26_RS16295 2945780..2946523 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T78386 WP_000244772.1 NZ_CP022086:c2941563-2941156 [Escherichia coli O104:H4]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T78386 NZ_CP022086:c2941563-2941156 [Escherichia coli O104:H4]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT78386 WP_000354046.1 NZ_CP022086:c2941810-2941544 [Escherichia coli O104:H4]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT78386 NZ_CP022086:c2941810-2941544 [Escherichia coli O104:H4]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References