Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2804849..2805432 Replicon chromosome
Accession NZ_CP022086
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag CEQ26_RS15615 Protein ID WP_000254745.1
Coordinates 2804849..2805184 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag CEQ26_RS15620 Protein ID WP_000581937.1
Coordinates 2805184..2805432 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS15600 2800736..2802034 - 1299 WP_000036723.1 phosphopyruvate hydratase -
CEQ26_RS15605 2802122..2803759 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
CEQ26_RS15610 2803987..2804778 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
CEQ26_RS15615 2804849..2805184 - 336 WP_000254745.1 endoribonuclease MazF Toxin
CEQ26_RS15620 2805184..2805432 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CEQ26_RS15625 2805510..2807744 - 2235 WP_000226815.1 GTP pyrophosphokinase -
CEQ26_RS15630 2807792..2809093 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T78385 WP_000254745.1 NZ_CP022086:c2805184-2804849 [Escherichia coli O104:H4]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T78385 NZ_CP022086:c2805184-2804849 [Escherichia coli O104:H4]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT78385 WP_000581937.1 NZ_CP022086:c2805432-2805184 [Escherichia coli O104:H4]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT78385 NZ_CP022086:c2805432-2805184 [Escherichia coli O104:H4]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References