Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2496277..2496902 Replicon chromosome
Accession NZ_CP022086
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CEQ26_RS14055 Protein ID WP_000911329.1
Coordinates 2496277..2496675 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CEQ26_RS14060 Protein ID WP_000450524.1
Coordinates 2496675..2496902 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS14035 2492155..2492355 + 201 WP_000383836.1 YpfN family protein -
CEQ26_RS14040 2492465..2493163 - 699 WP_000679823.1 esterase -
CEQ26_RS14045 2493237..2495252 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
CEQ26_RS14050 2495267..2496130 - 864 WP_001267498.1 neutral zinc metallopeptidase -
CEQ26_RS14055 2496277..2496675 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CEQ26_RS14060 2496675..2496902 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CEQ26_RS14065 2497057..2497770 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CEQ26_RS14070 2497983..2499017 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CEQ26_RS14075 2499034..2499912 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CEQ26_RS14080 2500058..2500630 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CEQ26_RS14085 2500630..2501100 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T78384 WP_000911329.1 NZ_CP022086:c2496675-2496277 [Escherichia coli O104:H4]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T78384 NZ_CP022086:c2496675-2496277 [Escherichia coli O104:H4]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT78384 WP_000450524.1 NZ_CP022086:c2496902-2496675 [Escherichia coli O104:H4]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT78384 NZ_CP022086:c2496902-2496675 [Escherichia coli O104:H4]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References