Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 828566..829384 Replicon chromosome
Accession NZ_CP022086
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0E0Y102
Locus tag CEQ26_RS05050 Protein ID WP_000771609.1
Coordinates 829010..829384 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0E0Y470
Locus tag CEQ26_RS05045 Protein ID WP_001285568.1
Coordinates 828566..828919 (+) Length 118 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS05005 823839..824189 + 351 WP_000624688.1 IS66 family insertion sequence element accessory protein TnpB -
CEQ26_RS05010 824220..825833 + 1614 WP_000080172.1 IS66-like element ISEc43 family transposase -
CEQ26_RS05020 826081..826902 + 822 WP_001241709.1 DUF945 domain-containing protein -
CEQ26_RS05025 826902..827147 + 246 WP_001164966.1 hypothetical protein -
CEQ26_RS05030 827241..827714 + 474 WP_000855049.1 antirestriction protein -
CEQ26_RS05035 827730..828206 + 477 WP_001186756.1 RadC family protein -
CEQ26_RS05040 828269..828490 + 222 WP_000692357.1 DUF987 domain-containing protein -
CEQ26_RS05045 828566..828919 + 354 WP_001285568.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
CEQ26_RS05050 829010..829384 + 375 WP_000771609.1 TA system toxin CbtA family protein Toxin
CEQ26_RS05055 829381..829872 + 492 WP_000976860.1 hypothetical protein -
CEQ26_RS05060 829884..830081 + 198 WP_000839270.1 DUF957 domain-containing protein -
CEQ26_RS05065 830166..831008 + 843 WP_001274560.1 DUF4942 domain-containing protein -
CEQ26_RS05075 831479..832417 + 939 WP_000351313.1 glyoxylate/hydroxypyruvate reductase GhrA -
CEQ26_RS05080 832472..833209 + 738 WP_000283664.1 zinc-binding phosphatase -
CEQ26_RS05085 833233..833787 + 555 WP_001001902.1 molecular chaperone YcdY -
CEQ26_RS05090 833889..834380 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 813544..838885 25341


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(13-112)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14006.09 Da        Isoelectric Point: 7.2652

>T78373 WP_000771609.1 NZ_CP022086:829010-829384 [Escherichia coli O104:H4]
MKLLPDTHVREASCCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Download         Length: 375 bp

>T78373 NZ_CP022086:829010-829384 [Escherichia coli O104:H4]
ATGAAATTATTACCCGACACACATGTACGGGAGGCATCATGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13064.82 Da        Isoelectric Point: 5.9541

>AT78373 WP_001285568.1 NZ_CP022086:828566-828919 [Escherichia coli O104:H4]
VSDTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPR
HQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Download         Length: 354 bp

>AT78373 NZ_CP022086:828566-828919 [Escherichia coli O104:H4]
GTGTCAGACACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGCGC
CCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATG
CGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCCCGC
CATCAGCATACCGTCACGCTATATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCT
GGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y102

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y470

References