Detailed information of TA system TA078371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 19368..20166
• Replicon: plasmid unnamed1
• Accession: NZ_CP022085
• Organism: Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: CEQ26_RS00120
• Protein ID: WP_014962311.1
• Coordinates: 19645..20166 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: CEQ26_RS00115
• Protein ID: WP_001351987.1
• Coordinates: 19368..19637 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ26_RS00095	15355..16095	-	741	WP_014962309.1	hypothetical protein	-
CEQ26_RS00100	16276..17970	-	1695	WP_014962310.1	hypothetical protein	-
CEQ26_RS00105	18022..18375	-	354	WP_160378290.1	hypothetical protein	-
CEQ26_RS00110	18381..19049	-	669	WP_000161228.1	AAA family ATPase	-
CEQ26_RS00115	19368..19637	+	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
CEQ26_RS00120	19645..20166	+	522	WP_014962311.1	GNAT family N-acetyltransferase	Toxin
CEQ26_RS00130	20334..20585	-	252	WP_001404395.1	hypothetical protein	-
CEQ26_RS00135	20587..21279	-	693	WP_000856758.1	membrane protein	-
CEQ26_RS00140	21293..21616	-	324	WP_014962312.1	hypothetical protein	-
CEQ26_RS00145	21714..22226	-	513	WP_014962313.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..109945	109945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19504.39 Da
• Isoelectric Point: 9.2612

>T78371 WP_014962311.1 NZ_CP022085:19645-20166 [Escherichia coli O104:H4]
VDNIKIEIFSGEKDYDLNGFDCGEESLNTFLTNHLKRQHEGKILRAYVLCTKKGRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T78371 NZ_CP022085:19645-20166 [Escherichia coli O104:H4]
GTGGATAATATAAAGATAGAAATTTTCTCTGGAGAGAAAGATTATGATTTGAATGGTTTTGATTGCGGTGAAGAATCACT
AAATACCTTTTTAACCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGCGCTTATGTGCTTTGCACGAAAAAAG
GCAGACCAAAAGTGTTAGGGTATTACACTTTGTCAGGTAGCTGCTTTGAAAAGGAGTCCTTACCTTCAAGAAGCCAGCAA
AAGAAGGTACCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGACTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT78371 WP_001351987.1 NZ_CP022085:19368-19637 [Escherichia coli O104:H4]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT78371 NZ_CP022085:19368-19637 [Escherichia coli O104:H4]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TGGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7