Detailed information of TA system TA078367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1855921..1856576
• Replicon: chromosome
• Accession: NZ_CP022084
• Organism: Burkholderia cepacia strain FDAARGOS_345

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2HGC0
• Locus tag: CEQ23_RS33265
• Protein ID: WP_021158162.1
• Coordinates: 1855921..1856340 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HMP5
• Locus tag: CEQ23_RS33270
• Protein ID: WP_021158161.1
• Coordinates: 1856337..1856576 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ23_RS33240	1851278..1852072	+	795	WP_027789489.1	AraC family transcriptional regulator	-
CEQ23_RS33245	1852332..1853285	+	954	WP_027789490.1	DMT family transporter	-
CEQ23_RS33250	1853470..1854018	+	549	WP_027789491.1	hypothetical protein	-
CEQ23_RS33255	1854122..1854256	-	135	WP_021158165.1	entericidin A/B family lipoprotein	-
CEQ23_RS33260	1854490..1855785	+	1296	WP_027789492.1	aspartate carbamoyltransferase	-
CEQ23_RS33265	1855921..1856340	-	420	WP_021158162.1	type II toxin-antitoxin system VapC family toxin	Toxin
CEQ23_RS33270	1856337..1856576	-	240	WP_021158161.1	antitoxin VapB	Antitoxin
CEQ23_RS33275	1856886..1859132	+	2247	WP_027789494.1	TonB-dependent siderophore receptor	-
CEQ23_RS33280	1859136..1859891	+	756	WP_027789495.1	sel1 repeat family protein	-
CEQ23_RS33285	1859888..1860571	+	684	WP_027789496.1	Fe2+-dependent dioxygenase	-
CEQ23_RS33290	1860660..1860899	+	240	WP_011659184.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CEQ23_RS33295	1860896..1861291	+	396	WP_027789497.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15212.57 Da
• Isoelectric Point: 5.9052

>T78367 WP_021158162.1 NZ_CP022084:c1856340-1855921 [Burkholderia cepacia]
MILVDTNVISEPLRREPNAAVIEWLDAQNVETLFLAAISLAEIRLGVAVLPEGRRREWLHQSIEQRVLPLFRGRILPFDD
AASKAYASLRARARAAGVAIAPSDGFIAGTAEANGLIVATRDVTPFEAMGIRVIDPWAR

Nucleotide

• Length: 420 bp

>T78367 NZ_CP022084:c1856340-1855921 [Burkholderia cepacia]
ATGATCCTTGTTGATACGAATGTCATTTCCGAACCGCTACGACGCGAGCCGAACGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAATGTCGAAACCTTGTTTCTGGCTGCGATCAGTCTCGCGGAAATACGATTGGGCGTGGCTGTATTGCCGGAAGGAC
GCCGGCGAGAGTGGCTGCATCAGAGCATCGAGCAGCGCGTGCTGCCATTGTTTCGCGGACGAATCCTGCCATTCGACGAT
GCTGCGAGCAAAGCGTATGCGAGCCTTCGCGCACGCGCTCGTGCCGCAGGTGTCGCAATTGCACCATCCGATGGTTTTAT
CGCCGGCACGGCCGAAGCGAACGGCCTGATCGTCGCCACGCGCGATGTCACGCCGTTCGAGGCAATGGGAATTCGCGTAA
TCGACCCGTGGGCGCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8629.79 Da
• Isoelectric Point: 5.6643

>AT78367 WP_021158161.1 NZ_CP022084:c1856576-1856337 [Burkholderia cepacia]
MPVITVRNLSDEVHRALRIRAAQHGRSTEAEVRDILEQAVLPGGRLKLGTMLAAIGQEAGGFDFDAQRDKTPADPMSFE

Nucleotide

• Length: 240 bp

>AT78367 NZ_CP022084:c1856576-1856337 [Burkholderia cepacia]
ATGCCGGTGATCACCGTCCGAAATCTGTCCGATGAAGTGCATCGAGCACTTCGCATTCGCGCGGCCCAACATGGGCGCAG
CACCGAAGCCGAGGTGCGCGACATTCTCGAACAGGCCGTTCTTCCGGGCGGGCGGCTCAAGCTGGGAACGATGCTGGCGG
CAATCGGGCAGGAGGCCGGCGGATTCGATTTCGACGCCCAGCGCGACAAGACGCCTGCCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: U2HGC0

Antitoxin

• AlphaFold DB: U2HMP5