Detailed information of TA system TA078363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 786993..787570
• Replicon: chromosome
• Accession: NZ_CP022084
• Organism: Burkholderia cepacia strain FDAARGOS_345

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2G063
• Locus tag: CEQ23_RS28400
• Protein ID: WP_021160897.1
• Coordinates: 786993..787358 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: CEQ23_RS28405
• Protein ID: WP_021160898.1
• Coordinates: 787352..787570 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ23_RS28380	782772..783326	+	555	WP_027791286.1	carboxymuconolactone decarboxylase family protein	-
CEQ23_RS41195	783648..784262	-	615	WP_155253146.1	hypothetical protein	-
CEQ23_RS28390	784367..785965	-	1599	WP_027791288.1	alkyl hydroperoxide reductase subunit F	-
CEQ23_RS28395	786091..786654	-	564	WP_006488685.1	peroxiredoxin	-
CEQ23_RS28400	786993..787358	-	366	WP_021160897.1	type II toxin-antitoxin system VapC family toxin	Toxin
CEQ23_RS28405	787352..787570	-	219	WP_021160898.1	hypothetical protein	Antitoxin
CEQ23_RS28410	787661..788557	-	897	WP_027791289.1	LysR family transcriptional regulator	-
CEQ23_RS28415	788735..789901	+	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
CEQ23_RS28420	789956..790480	+	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
CEQ23_RS28425	790473..791738	+	1266	WP_027791291.1	Zn-dependent hydrolase	-
CEQ23_RS28430	791826..792308	-	483	WP_027791292.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13448.50 Da
• Isoelectric Point: 8.4389

>T78363 WP_021160897.1 NZ_CP022084:c787358-786993 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSAQDEAPIRAWLSSFDVIALDSPVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T78363 NZ_CP022084:c787358-786993 [Burkholderia cepacia]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT78363 WP_021160898.1 NZ_CP022084:c787570-787352 [Burkholderia cepacia]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT78363 NZ_CP022084:c787570-787352 [Burkholderia cepacia]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: U2G063

Antitoxin

• AlphaFold DB: U2HEK9