Detailed information of TA system TA078358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4744568..4745180
• Replicon: chromosome
• Accession: NZ_CP022073
• Organism: Citrobacter koseri strain FDAARGOS_287

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: CEP66_RS23350
• Protein ID: WP_012906750.1
• Coordinates: 4744568..4744747 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CEP66_RS23355
• Protein ID: WP_047463070.1
• Coordinates: 4744770..4745180 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP66_RS23315	4739755..4741185	+	1431	WP_058668942.1	SDR family oxidoreductase	-
CEP66_RS23320	4741284..4742297	+	1014	WP_012133064.1	NAD(P)-dependent oxidoreductase	-
CEP66_RS23325	4742294..4743124	-	831	WP_058668941.1	N-acetylmuramoyl-L-alanine amidase	-
CEP66_RS23330	4743121..4743444	-	324	WP_012133087.1	heavy metal-binding domain-containing protein	-
CEP66_RS23335	4743770..4744009	+	240	WP_058667564.1	DinI family protein	-
CEP66_RS23340	4744015..4744245	+	231	WP_125337031.1	hypothetical protein	-
CEP66_RS23350	4744568..4744747	+	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
CEP66_RS23355	4744770..4745180	+	411	WP_047463070.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CEP66_RS23360	4745238..4746221	+	984	WP_058667560.1	glycosyltransferase	-
CEP66_RS23365	4746314..4746892	-	579	WP_058667558.1	tail fiber assembly protein	-
CEP66_RS23370	4746892..4748445	-	1554	WP_058668940.1	phage tail protein	-
CEP66_RS23375	4748438..4749001	-	564	WP_058668939.1	phage tail protein I	-
CEP66_RS23380	4748994..4749911	-	918	WP_060937433.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4742294..4784743	42449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T78358 WP_012906750.1 NZ_CP022073:4744568-4744747 [Citrobacter koseri]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T78358 NZ_CP022073:4744568-4744747 [Citrobacter koseri]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTAAAAGGCAGTCACCACCAGTT
CAAACACCCAACGAAGAAAGGGCTGGTAACGGTGAAACATCCACAAAAGGACATACCGCTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14887.79 Da
• Isoelectric Point: 4.3006

>AT78358 WP_047463070.1 NZ_CP022073:4744770-4745180 [Citrobacter koseri]
MLYPIAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESVREAIDAHIELLVEDGEAVPEATSVENWLDDPDYAGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Nucleotide

• Length: 411 bp

>AT78358 NZ_CP022073:4744770-4745180 [Citrobacter koseri]
ATGTTATATCCCATTGCTATTGATAAAGGCGATTCATCTTTCGGCGTTCGCGTACCGGATATTCCCGGCTGTTTTTCTGG
TGGTGATAACTACCAGGACGCAATCGAGAGTGTACGTGAAGCGATCGACGCACATATAGAATTACTGGTGGAAGATGGCG
AAGCAGTACCTGAAGCCACTAGTGTGGAAAACTGGCTGGACGATCCTGACTATGCTGGTGTGGTATGGGCGCTGGTGGAT
GTGGATGTTACGCGATTGATGGGGAAAGCTGAAAAAATCAATGTTACTCTTCCCTCTTTACTGATCCGACGTATCGACCA
GTTTGTCGCTGCACACCCTGAGTACGGTAGCCGCTCCGGCTTTCTGTCCCGTGTGGCGGCTGATAAGGTGATAGGACGAG
AAAAACGATAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4