Detailed information of TA system TA078347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3349350..3349942
• Replicon: chromosome
• Accession: NZ_CP022073
• Organism: Citrobacter koseri strain FDAARGOS_287

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CEP66_RS16480
• Protein ID: WP_058669399.1
• Coordinates: 3349350..3349604 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CEP66_RS16485
• Protein ID: WP_058669398.1
• Coordinates: 3349601..3349942 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP66_RS16460	3344445..3345908	+	1464	WP_058669401.1	xylulokinase	-
CEP66_RS16465	3345976..3347253	+	1278	WP_012131672.1	RbtT/DalT/CsbX family MFS transporter	-
CEP66_RS16470	3347302..3347964	+	663	WP_058669400.1	HAD-IA family hydrolase	-
CEP66_RS16475	3348043..3348942	-	900	WP_012131675.1	lipid kinase YegS	-
CEP66_RS16480	3349350..3349604	+	255	WP_058669399.1	type II toxin-antitoxin system HicA family toxin	Toxin
CEP66_RS16485	3349601..3349942	+	342	WP_058669398.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CEP66_RS16490	3350086..3350733	+	648	Protein_3083	phage portal protein	-
CEP66_RS16495	3351234..3353828	+	2595	WP_058669397.1	SIR2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-99)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9468.02 Da
• Isoelectric Point: 10.8834

>T78347 WP_058669399.1 NZ_CP022073:3349350-3349604 [Citrobacter koseri]
MNKRHLKTLSDVFARPIRSSIKWSDIESMLIALGAEIHEREGSRIAVALKGEKKIFHRPHPQPTTDKGAVNSLRIWLESL
GVTP

Nucleotide

• Length: 255 bp

>T78347 NZ_CP022073:3349350-3349604 [Citrobacter koseri]
ATGAATAAACGACACCTTAAAACGCTATCAGATGTGTTTGCCCGCCCAATCAGAAGCTCAATAAAGTGGTCTGATATTGA
GTCAATGCTTATTGCGTTGGGCGCGGAGATTCACGAACGAGAAGGTTCAAGGATTGCGGTTGCACTGAAAGGTGAGAAGA
AAATCTTTCACAGGCCACACCCGCAGCCAACCACTGATAAGGGGGCGGTAAACTCTCTTCGGATATGGCTTGAAAGTTTA
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12609.32 Da
• Isoelectric Point: 4.6229

>AT78347 WP_058669398.1 NZ_CP022073:3349601-3349942 [Citrobacter koseri]
MINVLKIDGHQAVITFDPEIEMFRGEFIGLNGGADFYAYSVEELKKEGAESLRIFLDECRKDGIEPYKSYSGKVTTRLTP
ERHEALTIAAQATGMSINEMLNEGVDLVIARHS

Nucleotide

• Length: 342 bp

>AT78347 NZ_CP022073:3349601-3349942 [Citrobacter koseri]
ATGATTAATGTACTTAAAATCGACGGCCATCAGGCCGTCATCACCTTTGACCCAGAAATCGAAATGTTTCGCGGTGAATT
TATTGGGCTTAACGGTGGTGCCGACTTCTACGCTTACAGCGTGGAAGAATTGAAAAAAGAAGGCGCTGAGTCGCTTCGTA
TCTTCCTGGATGAATGCAGAAAGGACGGTATTGAGCCTTATAAATCCTATAGCGGAAAAGTTACAACCCGTCTGACGCCT
GAGCGGCATGAAGCGTTGACTATTGCAGCGCAGGCTACAGGTATGTCTATTAATGAAATGCTGAATGAAGGTGTTGACCT
CGTTATCGCGCGTCATTCATAA