Detailed information of TA system TA078345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1414210..1414826
• Replicon: chromosome
• Accession: NZ_CP022073
• Organism: Citrobacter koseri strain FDAARGOS_287

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A427P7X6
• Locus tag: CEP66_RS06785
• Protein ID: WP_047459878.1
• Coordinates: 1414210..1414584 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CEP66_RS06790
• Protein ID: WP_160880417.1
• Coordinates: 1414584..1414826 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP66_RS06770	1411713..1412615	+	903	WP_012133916.1	formate dehydrogenase O subunit beta	-
CEP66_RS06775	1412612..1413247	+	636	WP_058668371.1	formate dehydrogenase cytochrome b556 subunit	-
CEP66_RS06780	1413244..1414173	+	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
CEP66_RS06785	1414210..1414584	-	375	WP_047459878.1	type II toxin-antitoxin system VapC family toxin	Toxin
CEP66_RS06790	1414584..1414826	-	243	WP_160880417.1	ribbon-helix-helix domain-containing protein	Antitoxin
CEP66_RS06795	1415062..1416003	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
CEP66_RS06800	1416048..1416485	-	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CEP66_RS06805	1416482..1417354	-	873	WP_012133924.1	virulence factor BrkB family protein	-
CEP66_RS06810	1417348..1417947	-	600	WP_058668370.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13799.04 Da
• Isoelectric Point: 9.4395

>T78345 WP_047459878.1 NZ_CP022073:c1414584-1414210 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRDEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T78345 NZ_CP022073:c1414584-1414210 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGATGAAGCGAAGAAGGCTCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATCGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTCATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTCGCGGGGATTCCGGGAGTCATTACGCCTTATCATTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9396.62 Da
• Isoelectric Point: 4.1961

>AT78345 WP_160880417.1 NZ_CP022073:c1414826-1414584 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW

Nucleotide

• Length: 243 bp

>AT78345 NZ_CP022073:c1414826-1414584 [Citrobacter koseri]
ATGATGGCTGGGATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTTAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAACTTCGCGAGGAGTGG
TGA

Structures

Toxin

• AlphaFold DB: A0A427P7X6