Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 273379..273999 Replicon chromosome
Accession NZ_CP022073
Organism Citrobacter koseri strain FDAARGOS_287

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CEP66_RS01310 Protein ID WP_002892050.1
Coordinates 273781..273999 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag CEP66_RS01305 Protein ID WP_012133514.1
Coordinates 273379..273753 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP66_RS01295 268515..269708 + 1194 WP_047458420.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CEP66_RS01300 269731..272877 + 3147 WP_012133513.1 efflux RND transporter permease AcrB -
CEP66_RS01305 273379..273753 + 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
CEP66_RS01310 273781..273999 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CEP66_RS01315 274183..274734 + 552 WP_049009805.1 maltose O-acetyltransferase -
CEP66_RS01320 274848..275318 + 471 WP_060815856.1 hypothetical protein -
CEP66_RS01325 275396..275536 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
CEP66_RS01330 275538..275798 - 261 WP_012133519.1 type B 50S ribosomal protein L31 -
CEP66_RS01335 276024..277574 + 1551 WP_058668708.1 EAL domain-containing protein -
CEP66_RS01340 277614..277967 - 354 WP_012133521.1 DUF1428 family protein -
CEP66_RS01345 278047..278661 - 615 WP_012133522.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 273379..285324 11945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T78343 WP_002892050.1 NZ_CP022073:273781-273999 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T78343 NZ_CP022073:273781-273999 [Citrobacter koseri]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT78343 WP_012133514.1 NZ_CP022073:273379-273753 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT78343 NZ_CP022073:273379-273753 [Citrobacter koseri]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACCAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References