Detailed information of TA system TA078308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1389941..1390470
• Replicon: chromosome
• Accession: NZ_CP022056
• Organism: Staphylococcus saprophyticus strain FDAARGOS_336

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q49Z22
• Locus tag: CEQ14_RS06895
• Protein ID: WP_011302703.1
• Coordinates: 1390108..1390470 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q49Z23
• Locus tag: CEQ14_RS06890
• Protein ID: WP_011302702.1
• Coordinates: 1389941..1390111 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ14_RS06865	1385672..1386151	+	480	WP_011302698.1	PH domain-containing protein	-
CEQ14_RS06870	1386144..1387682	+	1539	WP_069795158.1	PH domain-containing protein	-
CEQ14_RS06875	1387675..1388175	+	501	WP_011302700.1	PH domain-containing protein	-
CEQ14_RS06880	1388239..1388589	+	351	WP_002482750.1	holo-ACP synthase	-
CEQ14_RS06885	1388705..1389853	+	1149	WP_011302701.1	alanine racemase	-
CEQ14_RS06890	1389941..1390111	+	171	WP_011302702.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CEQ14_RS06895	1390108..1390470	+	363	WP_011302703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEQ14_RS06900	1390807..1391808	+	1002	WP_011302704.1	PP2C family protein-serine/threonine phosphatase	-
CEQ14_RS06905	1391887..1392213	+	327	WP_011302705.1	anti-sigma factor antagonist	-
CEQ14_RS06910	1392215..1392697	+	483	WP_002482755.1	anti-sigma B factor RsbW	-
CEQ14_RS06915	1392672..1393442	+	771	WP_002482756.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13482.70 Da
• Isoelectric Point: 10.3173

>T78308 WP_011302703.1 NZ_CP022056:1390108-1390470 [Staphylococcus saprophyticus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSDKKMKEVNNAIDISLGLHTIRSHKA

Nucleotide

• Length: 363 bp

>T78308 NZ_CP022056:1390108-1390470 [Staphylococcus saprophyticus]
ATGATGAGACGTGGTGACGTTTATTTAGCTGATTTATCACCAGTCCAGGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
AATTATACAAAACGATACTGGTAATAAATATAGTCCTACCGTAATTGTAGCCGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAATTAGATAAAGATTCTGTTATATTGCTAGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACTTATCTTTCTGATAAAAAAATGAAAGAAGTTAATAATGCCATTGA
TATTAGTTTAGGATTACATACTATTAGGAGTCACAAAGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6332.00 Da
• Isoelectric Point: 4.0121

>AT78308 WP_011302702.1 NZ_CP022056:1389941-1390111 [Staphylococcus saprophyticus]
MASFNQSRIHNIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT78308 NZ_CP022056:1389941-1390111 [Staphylococcus saprophyticus]
ATGGCAAGTTTCAACCAAAGTAGAATTCATAATATAGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGATTTGAA
CCTCTCCCTCGCAGCAGAAGCATTTCCGTTTGAATGTGAAGCTTGTGATTGCAACGAAGTATATATTAGCTCCAAGAATA
ATAATGAATGA

Structures

Toxin

• AlphaFold DB: Q49Z22

Antitoxin

• AlphaFold DB: Q49Z23