78301

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 71846..72371 Replicon plasmid unnamed2
Accession NZ_CP022052
Organism Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CEP72_RS30310 Protein ID WP_001159868.1
Coordinates 71846..72151 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CEP72_RS30315 Protein ID WP_000813634.1
Coordinates 72153..72371 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP72_RS30285 67049..68215 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CEP72_RS30290 68803..69141 - 339 WP_071525396.1 RepB family plasmid replication initiator protein -
CEP72_RS30295 69103..70316 + 1214 WP_162829202.1 IS3 family transposase -
CEP72_RS30305 71039..71845 - 807 WP_000016989.1 site-specific integrase -
CEP72_RS30310 71846..72151 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CEP72_RS30315 72153..72371 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CEP72_RS30320 72830..73513 + 684 WP_010891291.1 DNA-binding protein -
CEP72_RS30325 73510..74130 + 621 WP_001248529.1 hypothetical protein -
CEP72_RS30330 74127..74327 + 201 WP_000708307.1 hypothetical protein -
CEP72_RS30340 74735..75712 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
CEP72_RS30345 75997..76737 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
CEP72_RS30350 76858..77088 - 231 WP_032171660.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / espP / toxB / hlyD / hlyB / hlyA / hlyC / exeG / exeE 1..95288 95288


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T78301 WP_001159868.1 NZ_CP022052:c72151-71846 [Escherichia coli O157]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T78301 NZ_CP022052:c72151-71846 [Escherichia coli O157]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT78301 WP_000813634.1 NZ_CP022052:c72371-72153 [Escherichia coli O157]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT78301 NZ_CP022052:c72371-72153 [Escherichia coli O157]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References