Detailed information of TA system TA078300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 28524..29083
• Replicon: plasmid unnamed2
• Accession: NZ_CP022052
• Organism: Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: CEP72_RS30015
• Protein ID: WP_000421248.1
• Coordinates: 28524..28799 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: CEP72_RS30020
• Protein ID: WP_001178089.1
• Coordinates: 28799..29083 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP72_RS29980	23877..24266	-	390	WP_001172748.1	cytochrome b562 family protein	-
CEP72_RS29985	24310..26520	-	2211	WP_000592771.1	catalase/peroxidase KatP	-
CEP72_RS29990	26697..26882	+	186	WP_001302179.1	hypothetical protein	-
CEP72_RS29995	26882..27298	+	417	Protein_21	IS91 family transposase	-
CEP72_RS30000	27298..27864	+	567	Protein_22	IS3-like element IS600 family transposase	-
CEP72_RS30005	27908..28123	+	216	Protein_23	transposase	-
CEP72_RS30010	28223..28429	-	207	WP_001105064.1	hypothetical protein	-
CEP72_RS30015	28524..28799	-	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEP72_RS30020	28799..29083	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
CEP72_RS30035	29995..30852	-	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
CEP72_RS30040	30845..30919	-	75	WP_001370046.1	RepA leader peptide Tap	-
CEP72_RS30050	31155..31409	-	255	WP_000083831.1	replication regulatory protein RepA	-
CEP72_RS30055	31649..31987	-	339	WP_000766796.1	hypothetical protein	-
CEP72_RS30060	32025..32234	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
CEP72_RS30065	32280..32741	-	462	WP_001233853.1	thermonuclease family protein	-
CEP72_RS30070	32986..33198	-	213	WP_001302189.1	ANR family transcriptional regulator	-
CEP72_RS30075	33331..33891	-	561	WP_000840472.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / espP / toxB / hlyD / hlyB / hlyA / hlyC / exeG / exeE	1..95288	95288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T78300 WP_000421248.1 NZ_CP022052:c28799-28524 [Escherichia coli O157]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T78300 NZ_CP022052:c28799-28524 [Escherichia coli O157]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT78300 WP_001178089.1 NZ_CP022052:c29083-28799 [Escherichia coli O157]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT78300 NZ_CP022052:c29083-28799 [Escherichia coli O157]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8