Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 15521..16165 Replicon plasmid unnamed1
Accession NZ_CP022051
Organism Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag CEP72_RS29740 Protein ID WP_000833471.1
Coordinates 15521..15703 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag CEP72_RS29745 Protein ID WP_000466317.1
Coordinates 15728..16165 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP72_RS29705 12909..13232 + 324 WP_000432877.1 hypothetical protein -
CEP72_RS29710 13273..13497 + 225 WP_000866040.1 hypothetical protein -
CEP72_RS29715 13593..13817 + 225 WP_000749828.1 hypothetical protein -
CEP72_RS29720 13868..14134 + 267 WP_000702249.1 hypothetical protein -
CEP72_RS29725 14124..14366 + 243 WP_001450803.1 hypothetical protein -
CEP72_RS29735 14806..15084 + 279 WP_000545936.1 hypothetical protein -
CEP72_RS29740 15521..15703 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
CEP72_RS29745 15728..16165 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CEP72_RS29750 16194..16898 - 705 WP_001279866.1 hypothetical protein -
CEP72_RS29755 16895..17656 - 762 WP_001097937.1 conjugal transfer protein TraL -
CEP72_RS29760 17666..18073 - 408 WP_001190619.1 hypothetical protein -
CEP72_RS29765 18419..18757 + 339 WP_000753495.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..42641 42641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T78299 WP_000833471.1 NZ_CP022051:15521-15703 [Escherichia coli O157]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T78299 NZ_CP022051:15521-15703 [Escherichia coli O157]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT78299 WP_000466317.1 NZ_CP022051:15728-16165 [Escherichia coli O157]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT78299 NZ_CP022051:15728-16165 [Escherichia coli O157]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGCTCTTATTCTGTTGACGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTTAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References