78298

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 13868..14366 Replicon plasmid unnamed1
Accession NZ_CP022051
Organism Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)


Gene name TsxA Uniprot ID A0A0K6A0X9
Locus tag CEP72_RS29720 Protein ID WP_000702249.1
Coordinates 13868..14134 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag CEP72_RS29725 Protein ID WP_001450803.1
Coordinates 14124..14366 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP72_RS29685 10225..11229 - 1005 WP_001208090.1 replication initiation protein -
CEP72_RS29705 12909..13232 + 324 WP_000432877.1 hypothetical protein -
CEP72_RS29710 13273..13497 + 225 WP_000866040.1 hypothetical protein -
CEP72_RS29715 13593..13817 + 225 WP_000749828.1 hypothetical protein -
CEP72_RS29720 13868..14134 + 267 WP_000702249.1 hypothetical protein Toxin
CEP72_RS29725 14124..14366 + 243 WP_001450803.1 hypothetical protein Antitoxin
CEP72_RS29735 14806..15084 + 279 WP_000545936.1 hypothetical protein -
CEP72_RS29740 15521..15703 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
CEP72_RS29745 15728..16165 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin -
CEP72_RS29750 16194..16898 - 705 WP_001279866.1 hypothetical protein -
CEP72_RS29755 16895..17656 - 762 WP_001097937.1 conjugal transfer protein TraL -
CEP72_RS29760 17666..18073 - 408 WP_001190619.1 hypothetical protein -
CEP72_RS29765 18419..18757 + 339 WP_000753495.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..42641 42641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)

Antitoxin

Toxin YoeB

(5-84)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10242.76 Da        Isoelectric Point: 9.9280

>T78298 WP_000702249.1 NZ_CP022051:13868-14134 [Escherichia coli O157]
MKIRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Download         Length: 267 bp

>T78298 NZ_CP022051:13868-14134 [Escherichia coli O157]
ATGAAGATACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTAATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT78298 WP_001450803.1 NZ_CP022051:14124-14366 [Escherichia coli O157]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT78298 NZ_CP022051:14124-14366 [Escherichia coli O157]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K6A0X9

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References