78297

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 5302576..5303047 Replicon chromosome
Accession NZ_CP022050
Organism Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag CEP72_RS28310 Protein ID WP_001303511.1
Coordinates 5302769..5303047 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag CEP72_RS28305 Protein ID WP_001302048.1
Coordinates 5302576..5302767 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP72_RS28265 5297783..5298367 - 585 WP_000206793.1 DUF551 domain-containing protein -
CEP72_RS28270 5298424..5298819 - 396 WP_001118159.1 DUF977 family protein -
CEP72_RS28275 5298835..5299604 - 770 Protein_5137 DUF1627 domain-containing protein -
CEP72_RS28280 5299630..5300370 - 741 WP_000788938.1 ATP-binding protein -
CEP72_RS28285 5300377..5301339 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
CEP72_RS28290 5301362..5301787 - 426 WP_000693943.1 Rha family transcriptional regulator -
CEP72_RS28295 5301784..5302086 - 303 WP_000172738.1 transcriptional regulator -
CEP72_RS28300 5302184..5302555 + 372 WP_001169686.1 hypothetical protein -
CEP72_RS28305 5302576..5302767 + 192 WP_001302048.1 hypothetical protein Antitoxin
CEP72_RS28310 5302769..5303047 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CEP72_RS28320 5303400..5303699 + 300 WP_001240334.1 hypothetical protein -
CEP72_RS28325 5303771..5303989 + 219 WP_001171930.1 hypothetical protein -
CEP72_RS28335 5304558..5304746 + 189 WP_000449192.1 cell division inhibition protein DicB -
CEP72_RS28340 5304743..5304934 + 192 WP_001090200.1 DUF1482 family protein -
CEP72_RS28345 5305027..5307498 + 2472 WP_000048458.1 exonuclease -
CEP72_RS28350 5307571..5307822 + 252 WP_000005552.1 excisionase family protein -
CEP72_RS31135 5307857..5308044 + 188 Protein_5151 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleC / nleG7' 5211212..5311334 100122


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T78297 WP_001303511.1 NZ_CP022050:5302769-5303047 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T78297 NZ_CP022050:5302769-5303047 [Escherichia coli O157]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT78297 WP_001302048.1 NZ_CP022050:5302576-5302767 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT78297 NZ_CP022050:5302576-5302767 [Escherichia coli O157]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
AlphaFold DB A0A829CTP0

References