Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2874093..2874893 Replicon chromosome
Accession NZ_CP022050
Organism Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag CEP72_RS14930 Protein ID WP_000342448.1
Coordinates 2874366..2874893 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag CEP72_RS14925 Protein ID WP_001277108.1
Coordinates 2874093..2874359 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP72_RS14905 2869751..2870419 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
CEP72_RS14910 2870412..2871470 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
CEP72_RS14915 2871715..2872569 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
CEP72_RS14920 2872840..2873943 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
CEP72_RS14925 2874093..2874359 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
CEP72_RS14930 2874366..2874893 + 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
CEP72_RS14935 2874890..2875273 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
CEP72_RS14940 2875697..2876806 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
CEP72_RS14945 2876854..2877780 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CEP72_RS14950 2877777..2879054 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
CEP72_RS14955 2879051..2879818 + 768 WP_000082104.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T78276 WP_000342448.1 NZ_CP022050:2874366-2874893 [Escherichia coli O157]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T78276 NZ_CP022050:2874366-2874893 [Escherichia coli O157]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT78276 WP_001277108.1 NZ_CP022050:2874093-2874359 [Escherichia coli O157]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT78276 NZ_CP022050:2874093-2874359 [Escherichia coli O157]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References