Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2831662..2832293 Replicon chromosome
Accession NZ_CP022050
Organism Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag CEP72_RS14680 Protein ID WP_001259386.1
Coordinates 2831662..2831937 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CEP72_RS14685 Protein ID WP_000593555.1
Coordinates 2831934..2832293 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP72_RS14665 2826666..2827733 + 1068 WP_000361458.1 HlyD family secretion protein -
CEP72_RS14670 2827730..2830465 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
CEP72_RS14675 2830465..2831589 + 1125 WP_088592024.1 ABC transporter permease -
CEP72_RS14680 2831662..2831937 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
CEP72_RS14685 2831934..2832293 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CEP72_RS14690 2832343..2833203 - 861 WP_001131788.1 class II aldolase -
CEP72_RS14695 2833235..2833504 - 270 WP_000933060.1 HPr family phosphocarrier protein -
CEP72_RS14700 2833494..2835002 - 1509 WP_001301773.1 carbohydrate kinase -
CEP72_RS14705 2834995..2836353 - 1359 Protein_2650 PTS galactitol transporter subunit IIC -
CEP72_RS14710 2836430..2836711 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
CEP72_RS14715 2836708..2837181 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2822806..2832293 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T78275 WP_001259386.1 NZ_CP022050:2831662-2831937 [Escherichia coli O157]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T78275 NZ_CP022050:2831662-2831937 [Escherichia coli O157]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT78275 WP_000593555.1 NZ_CP022050:2831934-2832293 [Escherichia coli O157]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT78275 NZ_CP022050:2831934-2832293 [Escherichia coli O157]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References