Detailed information of TA system TA078269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1615472..1616022
• Replicon: chromosome
• Accession: NZ_CP022050
• Organism: Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: CEP72_RS08565
• Protein ID: WP_001160975.1
• Coordinates: 1615472..1615786 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: CEP72_RS08570
• Protein ID: WP_000125566.1
• Coordinates: 1615789..1616022 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP72_RS08540	1611147..1612433	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
CEP72_RS08545	1612433..1613422	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CEP72_RS08550	1613419..1614240	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CEP72_RS08555	1614243..1614620	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CEP72_RS08560	1614627..1615475	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CEP72_RS08565	1615472..1615786	-	315	WP_001160975.1	CcdB family protein	Toxin
CEP72_RS08570	1615789..1616022	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CEP72_RS08575	1616108..1616587	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
CEP72_RS08580	1616779..1618641	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
CEP72_RS08585	1618634..1619164	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CEP72_RS08590	1619272..1620603	-	1332	WP_001183177.1	MFS transporter	-
CEP72_RS08595	1620661..1620948	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T78269 WP_001160975.1 NZ_CP022050:c1615786-1615472 [Escherichia coli O157]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T78269 NZ_CP022050:c1615786-1615472 [Escherichia coli O157]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT78269 WP_000125566.1 NZ_CP022050:c1616022-1615789 [Escherichia coli O157]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT78269 NZ_CP022050:c1616022-1615789 [Escherichia coli O157]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9