78269

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1615472..1616022 Replicon chromosome
Accession NZ_CP022050
Organism Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag CEP72_RS08565 Protein ID WP_001160975.1
Coordinates 1615472..1615786 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CEP72_RS08570 Protein ID WP_000125566.1
Coordinates 1615789..1616022 (-) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP72_RS08540 1611147..1612433 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CEP72_RS08545 1612433..1613422 + 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CEP72_RS08550 1613419..1614240 + 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CEP72_RS08555 1614243..1614620 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CEP72_RS08560 1614627..1615475 + 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CEP72_RS08565 1615472..1615786 - 315 WP_001160975.1 CcdB family protein Toxin
CEP72_RS08570 1615789..1616022 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CEP72_RS08575 1616108..1616587 - 480 WP_000624372.1 type 3 dihydrofolate reductase -
CEP72_RS08580 1616779..1618641 - 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
CEP72_RS08585 1618634..1619164 - 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CEP72_RS08590 1619272..1620603 - 1332 WP_001183177.1 MFS transporter -
CEP72_RS08595 1620661..1620948 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-103)

Antitoxin

(8-77)


Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T78269 WP_001160975.1 NZ_CP022050:c1615786-1615472 [Escherichia coli O157]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T78269 NZ_CP022050:c1615786-1615472 [Escherichia coli O157]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT78269 WP_000125566.1 NZ_CP022050:c1616022-1615789 [Escherichia coli O157]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT78269 NZ_CP022050:c1616022-1615789 [Escherichia coli O157]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References