Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3956109..3956729 Replicon chromosome
Accession NZ_CP022049
Organism Citrobacter braakii strain FDAARGOS_290

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CEP69_RS19295 Protein ID WP_002892050.1
Coordinates 3956109..3956327 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag CEP69_RS19300 Protein ID WP_003021733.1
Coordinates 3956355..3956729 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP69_RS19260 3951468..3952097 + 630 WP_016155872.1 membrane protein -
CEP69_RS19265 3952162..3952515 + 354 WP_003831033.1 DUF1428 family protein -
CEP69_RS19270 3952567..3954120 - 1554 Protein_3626 EAL domain-containing protein -
CEP69_RS19275 3954309..3954569 + 261 WP_016152070.1 type B 50S ribosomal protein L31 -
CEP69_RS19280 3954571..3954711 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
CEP69_RS19285 3954790..3955260 - 471 WP_016152071.1 YlaC family protein -
CEP69_RS19290 3955377..3955928 - 552 WP_016155874.1 maltose O-acetyltransferase -
CEP69_RS19295 3956109..3956327 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CEP69_RS19300 3956355..3956729 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
CEP69_RS19305 3957217..3960366 - 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
CEP69_RS19310 3960389..3961582 - 1194 WP_016152074.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T78245 WP_002892050.1 NZ_CP022049:c3956327-3956109 [Citrobacter braakii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T78245 NZ_CP022049:c3956327-3956109 [Citrobacter braakii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT78245 WP_003021733.1 NZ_CP022049:c3956729-3956355 [Citrobacter braakii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT78245 NZ_CP022049:c3956729-3956355 [Citrobacter braakii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACTAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References