Detailed information of TA system TA078244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3416149..3416725
• Replicon: chromosome
• Accession: NZ_CP022049
• Organism: Citrobacter braakii strain FDAARGOS_290

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A8I0KMP7
• Locus tag: CEP69_RS16790
• Protein ID: WP_016155613.1
• Coordinates: 3416149..3416436 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A2I8S1U6
• Locus tag: CEP69_RS16795
• Protein ID: WP_016155614.1
• Coordinates: 3416423..3416725 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP69_RS16750	3411634..3411798	+	165	WP_016155606.1	DUF1127 domain-containing protein	-
CEP69_RS16755	3411947..3412401	+	455	Protein_3149	MarR family transcriptional regulator	-
CEP69_RS16760	3412407..3412688	+	282	WP_016155608.1	hypothetical protein	-
CEP69_RS16765	3412804..3413166	+	363	WP_016155609.1	DUF2778 domain-containing protein	-
CEP69_RS16770	3413166..3413672	+	507	WP_016155610.1	hypothetical protein	-
CEP69_RS16775	3413673..3414164	+	492	WP_016151575.1	type VI secretion system tube protein Hcp	-
CEP69_RS16780	3414259..3414519	-	261	Protein_3154	endoribonuclease SymE	-
CEP69_RS23920	3414506..3414652	-	147	Protein_3155	type I restriction endonuclease subunit R	-
CEP69_RS16785	3414748..3415914	-	1167	WP_019078303.1	restriction endonuclease	-
CEP69_RS16790	3416149..3416436	+	288	WP_016155613.1	BrnT family toxin	Toxin
CEP69_RS16795	3416423..3416725	+	303	WP_016155614.1	BrnA antitoxin family protein	Antitoxin
CEP69_RS16800	3417026..3418873	-	1848	WP_016155615.1	AAA family ATPase	-
CEP69_RS16805	3419060..3420016	-	957	WP_016155616.1	GTPase	-
CEP69_RS16810	3420029..3420223	-	195	WP_003830038.1	YbdD/YjiX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11310.75 Da
• Isoelectric Point: 7.4687

>T78244 WP_016155613.1 NZ_CP022049:3416149-3416436 [Citrobacter braakii]
MPMEFEWDANKAQSNLRKHGVRFEDAILVFDDPQHLSRQERYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHS

Nucleotide

• Length: 288 bp

>T78244 NZ_CP022049:3416149-3416436 [Citrobacter braakii]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAGTAACCTGAGGAAACATGGTGTTCGCTTTGAGGACGCAAT
ATTGGTTTTCGACGATCCCCAACATCTGTCGCGGCAGGAGCGGTATGAGAACGGTGAGTATCGCTGGCAGACCATTGGTC
TCGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGC
GCCAGAAAGGCAGATCGTAAAGAGAGGAATCGTTATGAGCATAGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11466.12 Da
• Isoelectric Point: 10.1293

>AT78244 WP_016155614.1 NZ_CP022049:3416423-3416725 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT78244 NZ_CP022049:3416423-3416725 [Citrobacter braakii]
ATGAGCATAGTTAAACATAAACGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
TAAACCGGACGATGATATCGATTACAGCGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCCATTCGCGGTAAGT
TTTTTCGTCCGTTAAAAACGCAGGCGTCGGTGCGCATTGATGCCGATGTGATGGAATGGCTGAAGCGCCCCGGGAAGGGT
TACCAGACGCGTCTCAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A2I8S1U6