Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2863797..2864413 Replicon chromosome
Accession NZ_CP022049
Organism Citrobacter braakii strain FDAARGOS_290

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CEP69_RS14180 Protein ID WP_016155182.1
Coordinates 2864039..2864413 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R8WIS4
Locus tag CEP69_RS14175 Protein ID WP_016155181.1
Coordinates 2863797..2864039 (+) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP69_RS14140 2859205..2859804 + 600 WP_016157873.1 glucose-1-phosphatase -
CEP69_RS14145 2859798..2860670 + 873 WP_019077938.1 virulence factor BrkB family protein -
CEP69_RS14150 2860667..2861104 + 438 WP_016157874.1 D-tyrosyl-tRNA(Tyr) deacylase -
CEP69_RS14155 2861149..2862090 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
CEP69_RS14160 2862096..2862383 - 288 WP_016157876.1 type II toxin-antitoxin system RelE/ParE family toxin -
CEP69_RS14165 2862376..2862618 - 243 WP_016155179.1 type II toxin-antitoxin system ParD family antitoxin -
CEP69_RS14170 2862683..2863591 - 909 Protein_2674 alpha/beta hydrolase -
CEP69_RS14175 2863797..2864039 + 243 WP_016155181.1 ribbon-helix-helix domain-containing protein Antitoxin
CEP69_RS14180 2864039..2864413 + 375 WP_016155182.1 type II toxin-antitoxin system VapC family toxin Toxin
CEP69_RS14185 2864450..2865379 - 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
CEP69_RS14190 2865376..2866011 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
CEP69_RS14195 2866008..2866910 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2851348..2864413 13065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-113)

Antitoxin

(13-47)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 13678.84 Da        Isoelectric Point: 8.5373

>T78241 WP_016155182.1 NZ_CP022049:2864039-2864413 [Citrobacter braakii]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Download         Length: 375 bp

>T78241 NZ_CP022049:2864039-2864413 [Citrobacter braakii]
ATGGTCACAGGATCTGCGCTTTTTGATACCAACATCCTGATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGAGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATCGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.72 Da        Isoelectric Point: 4.3331

>AT78241 WP_016155181.1 NZ_CP022049:2863797-2864039 [Citrobacter braakii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Download         Length: 243 bp

>AT78241 NZ_CP022049:2863797-2864039 [Citrobacter braakii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCTGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGCGAGGAATGG
TAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB R8WIS4

References