Detailed information of TA system TA078239

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1835861..1836515
• Replicon: chromosome
• Accession: NZ_CP022049
• Organism: Citrobacter braakii strain FDAARGOS_290

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: CEP69_RS08985
• Protein ID: WP_016154348.1
• Coordinates: 1835861..1836268 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: CEP69_RS08990
• Protein ID: WP_016154349.1
• Coordinates: 1836249..1836515 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP69_RS08965	1831753..1833486	-	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-
CEP69_RS08970	1833492..1834205	-	714	WP_016154345.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
CEP69_RS08975	1834229..1835125	-	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
CEP69_RS08980	1835239..1835760	+	522	WP_016157414.1	flavodoxin FldB	-
CEP69_RS08985	1835861..1836268	-	408	WP_016154348.1	protein YgfX	Toxin
CEP69_RS08990	1836249..1836515	-	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
CEP69_RS09000	1836773..1837753	+	981	WP_016157415.1	tRNA-modifying protein YgfZ	-
CEP69_RS09005	1837833..1838492	-	660	WP_016154351.1	hemolysin III family protein	-
CEP69_RS09010	1838656..1838967	-	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
CEP69_RS09015	1839020..1839748	+	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
CEP69_RS09020	1839869..1841302	+	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T78239 WP_016154348.1 NZ_CP022049:c1836268-1835861 [Citrobacter braakii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T78239 NZ_CP022049:c1836268-1835861 [Citrobacter braakii]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGCTGCTCATTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TCCGTAGCCAGCGCCGAATCAATGCCTGTCAGGGCGAGATTCGGCTGTTGATGGACGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGACTATTGCTAGCGCACCCTGGATGGTCAAAACAGGCATGATGCTGCGTTTACGTTCCGATGCCGGAAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGACGCGGAGTGGCGGGATTTGCGGCGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT78239 WP_016154349.1 NZ_CP022049:c1836515-1836249 [Citrobacter braakii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT78239 NZ_CP022049:c1836515-1836249 [Citrobacter braakii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGATGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGCTCAGCTAGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6