Detailed information of TA system TA078238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 341230..341866
• Replicon: chromosome
• Accession: NZ_CP022049
• Organism: Citrobacter braakii strain FDAARGOS_290

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: CEP69_RS01690
• Protein ID: WP_049259794.1
• Coordinates: 341678..341866 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CEP69_RS01685
• Protein ID: WP_131382557.1
• Coordinates: 341230..341646 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP69_RS01665	336275..337219	-	945	WP_016153152.1	ABC transporter permease	-
CEP69_RS01670	337220..338236	-	1017	WP_016156582.1	ABC transporter ATP-binding protein	-
CEP69_RS01675	338253..339398	-	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
CEP69_RS01680	339728..341137	-	1410	WP_016156583.1	PLP-dependent aminotransferase family protein	-
CEP69_RS01685	341230..341646	-	417	WP_131382557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CEP69_RS01690	341678..341866	-	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
CEP69_RS01695	342130..343533	+	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
CEP69_RS01700	343533..344543	+	1011	WP_016153157.1	cytochrome d ubiquinol oxidase subunit II	-
CEP69_RS01705	344543..344668	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
CEP69_RS01710	344858..345088	+	231	WP_016156585.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T78238 WP_049259794.1 NZ_CP022049:c341866-341678 [Citrobacter braakii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T78238 NZ_CP022049:c341866-341678 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGCTTACAGTCATCATCAATATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.33 Da
• Isoelectric Point: 4.7119

>AT78238 WP_131382557.1 NZ_CP022049:c341646-341230 [Citrobacter braakii]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT78238 NZ_CP022049:c341646-341230 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCGCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
CCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCACTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATTGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9