Detailed information of TA system TA078233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 596185..596724
• Replicon: chromosome
• Accession: NZ_CP022046
• Organism: Mammaliicoccus sciuri strain FDAARGOS_285

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A418JBZ2
• Locus tag: CEP64_RS17055
• Protein ID: WP_048540867.1
• Coordinates: 596353..596724 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CEP64_RS17050
• Protein ID: WP_048540868.1
• Coordinates: 596185..596352 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP64_RS17025	592067..592546	+	480	WP_058591087.1	PH domain-containing protein	-
CEP64_RS17030	592536..594056	+	1521	WP_088592231.1	PH domain-containing protein	-
CEP64_RS17035	594046..594510	+	465	WP_058591085.1	PH domain-containing protein	-
CEP64_RS17040	594542..594904	+	363	WP_058591084.1	holo-ACP synthase	-
CEP64_RS17045	594952..596100	+	1149	WP_058591083.1	alanine racemase	-
CEP64_RS17050	596185..596352	+	168	WP_048540868.1	hypothetical protein	Antitoxin
CEP64_RS17055	596353..596724	+	372	WP_048540867.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEP64_RS17060	596821..597825	+	1005	WP_048540866.1	PP2C family protein-serine/threonine phosphatase	-
CEP64_RS17065	597930..598253	+	324	WP_025904513.1	anti-sigma factor antagonist	-
CEP64_RS17070	598258..598734	+	477	WP_025904514.1	anti-sigma B factor RsbW	-
CEP64_RS17075	598709..599479	+	771	WP_088592232.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13653.83 Da
• Isoelectric Point: 9.9193

>T78233 WP_048540867.1 NZ_CP022046:596353-596724 [Mammaliicoccus sciuri]
MRRGDVYLADLSPVTGSEQGGTRPVVIIQNDTGNRYSPTVIVAAITGKINKAKIPTHVEIEAAKYKLDRDSVILLEQIRT
IDKKRLKEKLTYLSDAKMKEVDIAIAISLNLTLQQKFDTLGNT

Nucleotide

• Length: 372 bp

>T78233 NZ_CP022046:596353-596724 [Mammaliicoccus sciuri]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCACCAGTTACAGGTTCTGAACAAGGGGGAACAAGACCAGTCGTCAT
TATCCAGAATGATACAGGTAATCGATATAGCCCGACTGTGATTGTTGCAGCAATTACTGGTAAGATAAATAAAGCCAAAA
TTCCCACTCACGTAGAAATTGAGGCAGCGAAATATAAATTAGACAGAGATTCCGTAATTTTGCTTGAACAAATCAGAACA
ATTGATAAAAAACGCTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATATCGCTATTGCCAT
CAGCCTCAATCTAACTTTGCAACAAAAATTTGATACATTAGGTAATACATAA

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6120.58 Da
• Isoelectric Point: 4.0121

>AT78233 WP_048540868.1 NZ_CP022046:596185-596352 [Mammaliicoccus sciuri]
MTSLTQNRTQSLEQTLKEGYRSMADLNLSLASEAYYSECEACDSNESHLASQQGE

Nucleotide

• Length: 168 bp

>AT78233 NZ_CP022046:596185-596352 [Mammaliicoccus sciuri]
ATGACATCTTTAACGCAAAATCGTACACAAAGTTTAGAACAAACACTGAAAGAAGGCTATCGTTCAATGGCCGATTTAAA
TCTCTCCCTTGCATCTGAAGCATACTATAGCGAATGCGAAGCTTGTGATTCAAATGAATCGCATTTAGCCTCTCAGCAAG
GTGAATAA

Structures

Toxin

• AlphaFold DB: A0A418JBZ2