Detailed information of TA system TA078223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 85571..86096
• Replicon: plasmid punamed2
• Accession: NZ_CP022035
• Organism: Salmonella enterica subsp. enterica serovar Onderstepoort str. SA20060086

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: LFZ35_RS25030
• Protein ID: WP_001159863.1
• Coordinates: 85791..86096 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: I3W3D6
• Locus tag: LFZ35_RS25025
• Protein ID: WP_015062698.1
• Coordinates: 85571..85789 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFZ35_RS25005	81555..81749	-	195	WP_015062704.1	hypothetical protein	-
LFZ35_RS26165	82070..82288	+	219	WP_015062703.1	KUP/HAK/KT family potassium transporter	-
LFZ35_RS25010	82435..83424	+	990	WP_088546712.1	AraC family transcriptional regulator	-
LFZ35_RS25015	84183..84599	-	417	WP_015062700.1	type II toxin-antitoxin system VapC family toxin	-
LFZ35_RS25020	84596..84826	-	231	WP_088546699.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LFZ35_RS25025	85571..85789	+	219	WP_015062698.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LFZ35_RS25030	85791..86096	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
LFZ35_RS25035	86098..86388	+	291	WP_001266176.1	hypothetical protein	-
LFZ35_RS25040	86385..86911	+	527	Protein_87	cytoplasmic protein	-
LFZ35_RS25045	86924..87706	+	783	WP_015062695.1	site-specific integrase	-
LFZ35_RS25050	87857..88786	-	930	WP_015062694.1	Rpn family recombination-promoting nuclease/putative transposase	-
LFZ35_RS25055	89233..89523	-	291	WP_088546700.1	hypothetical protein	-
LFZ35_RS25060	89531..89797	-	267	WP_001749326.1	hypothetical protein	-
LFZ35_RS25065	89811..90098	-	288	WP_015062691.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LFZ35_RS25070	90095..90346	-	252	WP_015062690.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LFZ35_RS25075	90535..90765	-	231	WP_015062689.1	hypothetical protein	-
LFZ35_RS25080	90859..91050	-	192	WP_015062688.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..136771	136771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T78223 WP_001159863.1 NZ_CP022035:85791-86096 [Salmonella enterica subsp. enterica serovar Onderstepoort str.]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T78223 NZ_CP022035:85791-86096 [Salmonella enterica subsp. enterica serovar Onderstepoort str.]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT78223 WP_015062698.1 NZ_CP022035:85571-85789 [Salmonella enterica subsp. enterica serovar Onderstepoort str.]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT78223 NZ_CP022035:85571-85789 [Salmonella enterica subsp. enterica serovar Onderstepoort str.]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: I3W3D6