Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1842723..1843348 Replicon chromosome
Accession NZ_CP022023
Organism Klebsiella pneumoniae strain 19051

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CDA51_RS09130 Protein ID WP_002882817.1
Coordinates 1842965..1843348 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CDA51_RS09125 Protein ID WP_004150355.1
Coordinates 1842723..1842965 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDA51_RS09100 1838714..1839313 + 600 WP_004151865.1 glucose-1-phosphatase -
CDA51_RS09105 1839307..1840167 + 861 WP_004146232.1 virulence factor BrkB family protein -
CDA51_RS09110 1840164..1840601 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
CDA51_RS09115 1840646..1841587 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CDA51_RS09120 1841601..1842518 - 918 WP_004181612.1 alpha/beta hydrolase -
CDA51_RS09125 1842723..1842965 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
CDA51_RS09130 1842965..1843348 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CDA51_RS09135 1843522..1844451 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CDA51_RS09140 1844448..1845083 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CDA51_RS09145 1845080..1845982 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T78205 WP_002882817.1 NZ_CP022023:1842965-1843348 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T78205 NZ_CP022023:1842965-1843348 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT78205 WP_004150355.1 NZ_CP022023:1842723-1842965 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT78205 NZ_CP022023:1842723-1842965 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References