Detailed information of TA system TA078201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 110977..111554
• Replicon: plasmid pCFSAN021445
• Accession: NZ_CP022021
• Organism: Listeria monocytogenes strain FDA709873-33

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: ARX15_RS16365
• Protein ID: WP_003728465.1
• Coordinates: 110977..111318 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: ARX15_RS16370
• Protein ID: WP_003728464.1
• Coordinates: 111312..111554 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARX15_RS16350	107456..108010	-	555	WP_003728468.1	recombinase family protein	-
ARX15_RS16355	108290..108649	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
ARX15_RS16360	108649..110784	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
ARX15_RS16365	110977..111318	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ARX15_RS16370	111312..111554	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ARX15_RS16375	111615..111866	-	252	WP_003728463.1	hypothetical protein	-
ARX15_RS16380	111997..112323	+	327	WP_003728462.1	hypothetical protein	-
ARX15_RS16385	112323..112985	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
ARX15_RS16390	113004..113381	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
ARX15_RS16785	113540..114028	+	489	WP_003728520.1	hypothetical protein	-
ARX15_RS16400	114150..114398	+	249	WP_003728519.1	hypothetical protein	-
ARX15_RS16405	114399..114824	+	426	WP_003728518.1	hypothetical protein	-
ARX15_RS16410	114828..115550	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
ARX15_RS16415	115566..116281	+	716	Protein_125	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..152337	152337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T78201 WP_003728465.1 NZ_CP022021:c111318-110977 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T78201 NZ_CP022021:c111318-110977 [Listeria monocytogenes]
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Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT78201 WP_003728464.1 NZ_CP022021:c111554-111312 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT78201 NZ_CP022021:c111554-111312 [Listeria monocytogenes]
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TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
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TTT

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5