Detailed information of TA system TA078200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 272327..272917
• Replicon: plasmid unnamed1
• Accession: NZ_CP022016
• Organism: Salmonella enterica subsp. enterica serovar India str. SA20085604

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LFZ16_RS28600
• Protein ID: WP_223353822.1
• Coordinates: 272327..272659 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LFZ16_RS28605
• Protein ID: WP_223353824.1
• Coordinates: 272660..272917 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFZ16_RS28570	268091..268264	+	174	WP_223353814.1	hypothetical protein	-
LFZ16_RS28575 (LFZ16_29560)	268339..270159	+	1821	WP_223353816.1	ABC transporter ATP-binding protein	-
LFZ16_RS28580	270171..270671	+	501	WP_223353818.1	hypothetical protein	-
LFZ16_RS28585 (LFZ16_29565)	270673..271407	+	735	WP_223353820.1	S8 family serine peptidase	-
LFZ16_RS28590 (LFZ16_29570)	271411..271832	+	422	Protein_315	GDP-mannose 4,6-dehydratase	-
LFZ16_RS28595 (LFZ16_29575)	271853..272044	+	192	Protein_316	transposase	-
LFZ16_RS28600 (LFZ16_29580)	272327..272659	-	333	WP_223353822.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LFZ16_RS28605 (LFZ16_29585)	272660..272917	-	258	WP_223353824.1	antitoxin	Antitoxin
LFZ16_RS28610 (LFZ16_29595)	273529..277428	+	3900	WP_223353826.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	sopE2 / flhA / flhB / cheZ / cheY / cheB / cheR / cheW / cheA / motB / motA / flhC / flhD / fliA / fliS / fliG / fliI / fliM / fliN / fliP / fliQ / fliR / rcsA / sopA / sopA / ugd / gnd / ddhB / ddhA / galF / wcaJ / manB / wcaG / gmd / wza	1..473456	473456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11703.48 Da
• Isoelectric Point: 8.5572

>T78200 WP_223353822.1 NZ_CP022016:c272659-272327 [Salmonella enterica subsp. enterica serovar India str. SA20085604]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKRLECIPEAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T78200 NZ_CP022016:c272659-272327 [Salmonella enterica subsp. enterica serovar India str. SA20085604]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTGCTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGCTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAAACTTTGCCCGTG
CAGCTGGTTTTACCGTCTCATTGGACGGCGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGATCAGCCCAGAACCATT
GATATGGGGGCTCGGAACGGTAAGCGTTTGGAATGTATACCTGAAGCTGTCGTAAATGAAGTGCTGGCCCGATTGGAAGC
CATTCTAAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9363.81 Da
• Isoelectric Point: 4.2950

>AT78200 WP_223353824.1 NZ_CP022016:c272917-272660 [Salmonella enterica subsp. enterica serovar India str. SA20085604]
MYTTRLKKVGGSVMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPYAEMSDEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT78200 NZ_CP022016:c272917-272660 [Salmonella enterica subsp. enterica serovar India str. SA20085604]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTAGCGCT
GTCGACAGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCATTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGTACGCCGAAATGAGCGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA