Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2865282..2865919 Replicon chromosome
Accession NZ_CP022014
Organism Mycobacterium tuberculosis strain MTB2

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag B7R63_RS13925 Protein ID WP_003413180.1
Coordinates 2865282..2865677 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag B7R63_RS13930 Protein ID WP_003413183.1
Coordinates 2865674..2865919 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7R63_RS13880 2860686..2861897 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
B7R63_RS13890 2862024..2862683 + 660 WP_003902296.1 hypothetical protein -
B7R63_RS13895 2862680..2863342 + 663 WP_003905878.1 hypothetical protein -
B7R63_RS21545 2863339..2863616 + 278 Protein_2627 type II toxin-antitoxin system VapB family antitoxin -
B7R63_RS13905 2863709..2864122 + 414 WP_003413164.1 PIN domain nuclease -
B7R63_RS13910 2864161..2864418 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
B7R63_RS13915 2864415..2864792 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
B7R63_RS13920 2864808..2865182 - 375 WP_003413177.1 hypothetical protein -
B7R63_RS13925 2865282..2865677 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
B7R63_RS13930 2865674..2865919 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
B7R63_RS13940 2866330..2866749 - 420 WP_003413190.1 A24 family peptidase -
B7R63_RS13945 2866761..2867570 - 810 WP_003413193.1 shikimate dehydrogenase -
B7R63_RS13950 2867567..2868817 - 1251 WP_024753809.1 endolytic transglycosylase MltG -
B7R63_RS13955 2868810..2869322 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T78161 WP_003413180.1 NZ_CP022014:c2865677-2865282 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T78161 NZ_CP022014:c2865677-2865282 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT78161 WP_003413183.1 NZ_CP022014:c2865919-2865674 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT78161 NZ_CP022014:c2865919-2865674 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References