Detailed information of TA system TA078146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2175014..2175717
• Replicon: chromosome
• Accession: NZ_CP022014
• Organism: Mycobacterium tuberculosis strain MTB2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: B7R63_RS10410
• Protein ID: WP_003409778.1
• Coordinates: 2175014..2175343 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: B7R63_RS10415
• Protein ID: WP_003409780.1
• Coordinates: 2175340..2175717 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7R63_RS10390	2171397..2172467	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
B7R63_RS10395	2172464..2172979	+	516	WP_003409718.1	flavin reductase family protein	-
B7R63_RS10400	2172976..2174037	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
B7R63_RS10405	2174034..2174804	+	771	WP_003409775.1	SDR family oxidoreductase	-
B7R63_RS10410	2175014..2175343	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7R63_RS10415	2175340..2175717	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
B7R63_RS10420	2175714..2176304	-	591	WP_003409784.1	SEC-C domain-containing protein	-
B7R63_RS10425	2176359..2177723	+	1365	WP_003903691.1	HNH endonuclease	-
B7R63_RS10430	2177878..2178330	-	453	WP_003899095.1	lipoprotein	-
B7R63_RS10435	2178394..2178795	+	402	WP_003409869.1	hypothetical protein	-
B7R63_RS10440	2178788..2178970	-	183	WP_019250140.1	hypothetical protein	-
B7R63_RS10445	2179084..2179434	-	351	WP_003409871.1	hypothetical protein	-
B7R63_RS10450	2179445..2180347	-	903	WP_003899097.1	hypothetical protein	-
B7R63_RS10455	2180368..2180559	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T78146 WP_003409778.1 NZ_CP022014:c2175343-2175014 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T78146 NZ_CP022014:c2175343-2175014 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT78146 WP_003409780.1 NZ_CP022014:c2175717-2175340 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT78146 NZ_CP022014:c2175717-2175340 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8