Detailed information of TA system TA078137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1237313..1237881
• Replicon: chromosome
• Accession: NZ_CP022014
• Organism: Mycobacterium tuberculosis strain MTB2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: B7R63_RS06060
• Protein ID: WP_003405865.1
• Coordinates: 1237507..1237881 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: B7R63_RS06055
• Protein ID: WP_003405863.1
• Coordinates: 1237313..1237510 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7R63_RS06035	1233354..1233992	-	639	WP_003898730.1	lipid droplet-associated protein	-
B7R63_RS06040	1234082..1235089	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
B7R63_RS06045	1235106..1236089	-	984	WP_003898731.1	hypothetical protein	-
B7R63_RS06050	1236152..1237225	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
B7R63_RS06055	1237313..1237510	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B7R63_RS06060	1237507..1237881	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7R63_RS06065	1238084..1238782	+	699	WP_003898733.1	hypothetical protein	-
B7R63_RS06070	1238900..1239085	+	186	WP_003901093.1	hypothetical protein	-
B7R63_RS06075	1239012..1239287	-	276	WP_003405867.1	hypothetical protein	-
B7R63_RS06080	1239530..1239853	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
B7R63_RS06085	1239868..1240578	-	711	WP_003911399.1	hypothetical protein	-
B7R63_RS21775	1240600..1240809	+	210	WP_003911400.1	hypothetical protein	-
B7R63_RS06090	1240761..1241468	-	708	Protein_1158	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T78137 WP_003405865.1 NZ_CP022014:1237507-1237881 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T78137 NZ_CP022014:1237507-1237881 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT78137 WP_003405863.1 NZ_CP022014:1237313-1237510 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT78137 NZ_CP022014:1237313-1237510 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07