Detailed information of TA system TA078115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 73085..73718
• Replicon: chromosome
• Accession: NZ_CP022014
• Organism: Mycobacterium tuberculosis strain MTB2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B7R63_RS00370
• Protein ID: WP_003902592.1
• Coordinates: 73317..73718 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P0CW29
• Locus tag: B7R63_RS00365
• Protein ID: WP_003400577.1
• Coordinates: 73085..73324 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7R63_RS00350	68381..69841	+	1461	WP_003902591.1	FAD-binding oxidoreductase	-
B7R63_RS00355	69877..70038	+	162	WP_003899796.1	hypothetical protein	-
B7R63_RS00360	70079..73018	+	2940	WP_003901785.1	UPF0182 family protein	-
B7R63_RS00365	73085..73324	+	240	WP_003400577.1	antitoxin VapB1	Antitoxin
B7R63_RS00370	73317..73718	+	402	WP_003902592.1	type II toxin-antitoxin system ribonuclease VapC1	Toxin
B7R63_RS00375	73770..76007	-	2238	WP_003899797.1	NADP-dependent isocitrate dehydrogenase	-
B7R63_RS00380	76125..76694	-	570	WP_003400591.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
B7R63_RS00385	76797..77708	+	912	WP_003899798.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (24-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14349.48 Da
• Isoelectric Point: 4.8887

>T78115 WP_003902592.1 NZ_CP022014:73317-73718 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKEPISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Nucleotide

• Length: 402 bp

>T78115 NZ_CP022014:73317-73718 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGCCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8717.84 Da
• Isoelectric Point: 8.5960

>AT78115 WP_003400577.1 NZ_CP022014:73085-73324 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Nucleotide

• Length: 240 bp

>AT78115 NZ_CP022014:73085-73324 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHM7