Detailed information of TA system TA078099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 12432..12985
• Replicon: plasmid pls2
• Accession: NZ_CP022008
• Organism: Burkholderia gladioli pv. gladioli strain KACC 11889

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CEJ98_RS38010
• Protein ID: WP_080742290.1
• Coordinates: 12692..12985 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A808W488
• Locus tag: CEJ98_RS38005
• Protein ID: WP_042286915.1
• Coordinates: 12432..12704 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEJ98_RS37980	8153..8686	-	534	WP_042286924.1	PAAR domain-containing protein	-
CEJ98_RS37985	8723..9382	-	660	WP_052409305.1	hypothetical protein	-
CEJ98_RS37990	9500..9802	-	303	WP_042286921.1	hypothetical protein	-
CEJ98_RS39420	9799..10299	-	501	WP_144417590.1	hypothetical protein	-
CEJ98_RS37995	10374..10682	+	309	WP_042286918.1	MobB mobilization protein	-
CEJ98_RS38000	10679..12352	+	1674	WP_063769171.1	relaxase/mobilization nuclease domain-containing protein	-
CEJ98_RS38005	12432..12704	+	273	WP_042286915.1	CopG family ribbon-helix-helix protein	Antitoxin
CEJ98_RS38010	12692..12985	+	294	WP_080742290.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEJ98_RS38015	12982..13302	+	321	WP_042286911.1	hypothetical protein	-
CEJ98_RS38020	13575..13754	+	180	WP_042286906.1	hypothetical protein	-
CEJ98_RS38025	13744..14058	+	315	WP_042286903.1	hypothetical protein	-
CEJ98_RS38030	14092..14601	-	510	WP_042286898.1	hypothetical protein	-
CEJ98_RS38035	14613..16783	-	2171	Protein_21	DotA/TraY family protein	-
CEJ98_RS38040	16780..17388	-	609	WP_042286891.1	hypothetical protein	-
CEJ98_RS39830	17346..17630	-	285	WP_146775969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..26639	26639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11000.51 Da
• Isoelectric Point: 8.0198

>T78099 WP_080742290.1 NZ_CP022008:12692-12985 [Burkholderia gladioli pv. gladioli]
VPHVIITPRAGQGLERCRRFLADKSETAVRRAAQAIEQRFLLLETTPAVGRPFPENPAWRELAIPFGDSGYVALYRYEPA
DDAVYVLAFRHQREAGY

Nucleotide

• Length: 294 bp

>T78099 NZ_CP022008:12692-12985 [Burkholderia gladioli pv. gladioli]
GTGCCACACGTAATTATCACGCCTCGCGCCGGTCAAGGATTGGAGCGCTGCCGGCGCTTTCTTGCCGACAAAAGCGAGAC
AGCCGTTCGGCGGGCGGCGCAGGCCATCGAGCAGCGATTTCTATTGCTGGAAACGACGCCGGCCGTTGGCCGCCCTTTTC
CTGAAAATCCGGCGTGGCGCGAGCTGGCCATTCCGTTCGGCGACTCTGGCTATGTCGCCTTGTATCGGTACGAACCGGCA
GATGATGCCGTCTACGTGCTTGCTTTCCGGCATCAGAGAGAGGCGGGGTATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10456.58 Da
• Isoelectric Point: 5.1678

>AT78099 WP_042286915.1 NZ_CP022008:12432-12704 [Burkholderia gladioli pv. gladioli]
MATSLKIDDELKGRVQHLASTRDRSPHWIMCEAIRQYVEREEARESFKQEAAASWAHYQETGLHLTGEEVRTWLKTWGTD
DETGLPECHT

Nucleotide

• Length: 273 bp

>AT78099 NZ_CP022008:12432-12704 [Burkholderia gladioli pv. gladioli]
ATGGCAACGTCACTCAAGATCGACGACGAGCTGAAGGGGCGCGTACAGCACCTTGCCAGCACGCGTGATCGGTCGCCGCA
CTGGATTATGTGCGAGGCAATCAGGCAGTATGTCGAGCGCGAAGAAGCGCGCGAAAGCTTCAAGCAGGAGGCTGCGGCCT
CCTGGGCTCACTATCAGGAAACCGGCCTGCATCTGACCGGGGAAGAGGTTCGCACCTGGCTGAAAACGTGGGGCACGGAC
GATGAAACGGGGCTGCCTGAGTGCCACACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808W488