Detailed information of TA system TA078081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 4696886..4697522
• Replicon: chromosome
• Accession: NZ_CP022001
• Organism: Pseudomonas aeruginosa strain Pa1207

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CD800_RS22400
• Protein ID: WP_088540532.1
• Coordinates: 4696886..4697302 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CD800_RS22405
• Protein ID: WP_088540533.1
• Coordinates: 4697289..4697522 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD800_RS22375	4692189..4693127	+	939	WP_088540621.1	TIGR03756 family integrating conjugative element protein	-
CD800_RS22380	4693138..4694535	+	1398	WP_088540529.1	integrating conjugative element protein	-
CD800_RS22385	4694535..4694864	+	330	WP_088540530.1	hypothetical protein	-
CD800_RS22390	4694880..4696436	+	1557	WP_088540531.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
CD800_RS22395	4696446..4696889	-	444	WP_198346298.1	DUF3742 family protein	-
CD800_RS22400	4696886..4697302	-	417	WP_088540532.1	PIN domain-containing protein	Toxin
CD800_RS22405	4697289..4697522	-	234	WP_088540533.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CD800_RS22410	4697717..4698247	+	531	WP_088540534.1	NAD(P)-binding domain-containing protein	-
CD800_RS22415	4698257..4698838	+	582	WP_088540535.1	NAD/NADP octopine/nopaline dehydrogenase family protein	-
CD800_RS22420	4698894..4699775	+	882	WP_198346299.1	DMT family transporter	-
CD800_RS22425	4699820..4700716	-	897	WP_088540537.1	LysR family transcriptional regulator	-
CD800_RS22430	4700842..4702095	+	1254	WP_198346300.1	MmgE/PrpD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4654089..4767507	113418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15475.76 Da
• Isoelectric Point: 5.8946

>T78081 WP_088540532.1 NZ_CP022001:c4697302-4696886 [Pseudomonas aeruginosa]
MPEAKFKHFIDSNVVLYLLSSDAAKADSAEALLKTHPVISVQVLNEVTNVCLRKLHMSWDEIAQFLELVRSFCRVVPLTE
TIHDQARWVAEQHRLSFYDACIVAAAAAASCSTLYSEDMNHGQILEDSLTIRNPFVAK

Nucleotide

• Length: 417 bp

>T78081 NZ_CP022001:c4697302-4696886 [Pseudomonas aeruginosa]
ATGCCCGAGGCTAAGTTCAAGCACTTCATCGACAGCAATGTGGTGCTGTACCTGCTGTCGAGCGACGCGGCCAAGGCCGA
CAGCGCGGAGGCGCTGCTGAAGACTCACCCGGTCATCAGCGTGCAGGTGCTCAACGAGGTGACGAATGTGTGCTTGCGCA
AGTTGCACATGAGCTGGGATGAGATTGCCCAGTTCCTGGAACTGGTGCGCAGCTTCTGCCGGGTCGTGCCGCTCACGGAG
ACGATTCACGATCAGGCCCGGTGGGTGGCCGAACAGCACCGCCTGTCGTTCTACGATGCCTGCATCGTGGCGGCCGCAGC
AGCGGCCAGTTGCAGTACGCTCTACAGCGAAGACATGAACCATGGGCAGATACTGGAAGACAGCTTGACCATCAGGAATC
CGTTCGTGGCGAAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8543.76 Da
• Isoelectric Point: 7.0141

>AT78081 WP_088540533.1 NZ_CP022001:c4697522-4697289 [Pseudomonas aeruginosa]
MQVAKWGNSLAVRLPAALVDALELREGDDIEIVVDDPRTFAVRKKPGADALLERLRAFRGKLPADFHFSRDEANARG

Nucleotide

• Length: 234 bp

>AT78081 NZ_CP022001:c4697522-4697289 [Pseudomonas aeruginosa]
ATGCAAGTCGCCAAATGGGGTAACAGTCTCGCCGTGCGCCTTCCGGCTGCGCTGGTGGATGCGCTGGAGCTGCGTGAAGG
GGACGATATCGAGATCGTCGTGGATGATCCGCGCACGTTCGCGGTTCGCAAGAAACCTGGGGCCGATGCCTTGCTGGAGC
GCCTGCGCGCTTTCCGCGGCAAGCTGCCGGCAGATTTCCACTTCAGCCGTGACGAAGCCAATGCCCGAGGCTAA