Detailed information of TA system TA078080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4572674..4573716
• Replicon: chromosome
• Accession: NZ_CP022001
• Organism: Pseudomonas aeruginosa strain Pa1207

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CD800_RS21710
• Protein ID: WP_003153636.1
• Coordinates: 4572674..4573249 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CD800_RS21715
• Protein ID: WP_003050245.1
• Coordinates: 4573246..4573716 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD800_RS21685	4569112..4569837	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
CD800_RS21690	4569876..4570778	-	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
CD800_RS21695	4570778..4571278	-	501	WP_003090159.1	hypothetical protein	-
CD800_RS21700	4571275..4571745	-	471	WP_003153638.1	hypothetical protein	-
CD800_RS21705	4571742..4572656	-	915	WP_003050256.1	AAA family ATPase	-
CD800_RS21710	4572674..4573249	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
CD800_RS21715	4573246..4573716	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CD800_RS21720	4573920..4574303	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
CD800_RS21725	4574300..4574533	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
CD800_RS21730	4574550..4574909	+	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
CD800_RS21735	4574922..4575320	+	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
CD800_RS21740	4575317..4576009	+	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
CD800_RS21745	4576006..4576917	+	912	WP_034022488.1	TIGR03749 family integrating conjugative element protein	-
CD800_RS21750	4576907..4578325	+	1419	WP_003116808.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4550118..4651932	101814

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T78080 WP_003153636.1 NZ_CP022001:c4573249-4572674 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T78080 NZ_CP022001:c4573249-4572674 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT78080 WP_003050245.1 NZ_CP022001:c4573716-4573246 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT78080 NZ_CP022001:c4573716-4573246 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68