Detailed information of TA system TA078076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1810476..1811062
• Replicon: chromosome
• Accession: NZ_CP022001
• Organism: Pseudomonas aeruginosa strain Pa1207

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CD800_RS08610
• Protein ID: WP_003120987.1
• Coordinates: 1810476..1810775 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CD800_RS08615
• Protein ID: WP_003448662.1
• Coordinates: 1810772..1811062 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD800_RS08590	1805589..1806122	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
CD800_RS08595	1806112..1807437	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
CD800_RS08600	1807441..1809150	-	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
CD800_RS08605	1809150..1810274	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
CD800_RS08610	1810476..1810775	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CD800_RS08615	1810772..1811062	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CD800_RS08620	1811133..1811477	-	345	WP_016851612.1	hypothetical protein	-
CD800_RS08625	1811618..1813594	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
CD800_RS08630	1813591..1815480	-	1890	WP_016851610.1	hypothetical protein	-
CD800_RS08635	1815702..1815911	-	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1735211..1838035	102824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T78076 WP_003120987.1 NZ_CP022001:1810476-1810775 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T78076 NZ_CP022001:1810476-1810775 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT78076 WP_003448662.1 NZ_CP022001:1810772-1811062 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT78076 NZ_CP022001:1810772-1811062 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73