Detailed information of TA system TA078065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4926280..4926888
• Replicon: chromosome
• Accession: NZ_CP021999
• Organism: Pseudomonas aeruginosa strain Pa84

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: CD796_RS23720
• Protein ID: WP_042929582.1
• Coordinates: 4926280..4926627 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: CD796_RS23725
• Protein ID: WP_003114155.1
• Coordinates: 4926637..4926888 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD796_RS23690	4921639..4921911	+	273	WP_003120794.1	cysteine-rich CWC family protein	-
CD796_RS23695	4921911..4922603	+	693	WP_003098362.1	16S rRNA pseudouridine(516) synthase	-
CD796_RS23700	4922739..4923782	+	1044	WP_003098363.1	L,D-transpeptidase	-
CD796_RS23705	4923862..4924599	+	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
CD796_RS23710	4925051..4925953	+	903	WP_088002856.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
CD796_RS23720	4926280..4926627	-	348	WP_042929582.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CD796_RS23725	4926637..4926888	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CD796_RS23730	4927102..4928085	-	984	WP_031672657.1	tyrosine-type recombinase/integrase	-
CD796_RS23735	4928085..4929377	-	1293	WP_003115206.1	hypothetical protein	-
CD796_RS23740	4929607..4930881	-	1275	WP_042929584.1	zonular occludens toxin family protein	-
CD796_RS23745	4930885..4931241	-	357	WP_003114150.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	4926280..4949529	23249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12856.61 Da
• Isoelectric Point: 4.5143

>T78065 WP_042929582.1 NZ_CP021999:c4926627-4926280 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGAQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGGLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T78065 NZ_CP021999:c4926627-4926280 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGCACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGGGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT78065 WP_003114155.1 NZ_CP021999:c4926888-4926637 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT78065 NZ_CP021999:c4926888-4926637 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355