Detailed information of TA system TA078061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1591771..1592428
• Replicon: chromosome
• Accession: NZ_CP021999
• Organism: Pseudomonas aeruginosa strain Pa84

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: CD796_RS07665
• Protein ID: WP_003098540.1
• Coordinates: 1592246..1592428 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CD796_RS07660
• Protein ID: WP_003098541.1
• Coordinates: 1591771..1592199 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD796_RS07615	1586996..1587193	+	198	WP_042854192.1	hypothetical protein	-
CD796_RS07620	1587345..1588049	+	705	WP_088406678.1	phage regulatory protein/antirepressor Ant	-
CD796_RS07625	1588114..1588947	+	834	WP_031642905.1	helix-turn-helix domain-containing protein	-
CD796_RS07630	1588934..1589731	+	798	WP_023118140.1	hypothetical protein	-
CD796_RS07635	1589728..1589934	+	207	WP_023118139.1	hypothetical protein	-
CD796_RS07640	1589931..1590302	+	372	WP_003098545.1	recombinase	-
CD796_RS07645	1590299..1590646	+	348	WP_023118137.1	RusA family crossover junction endodeoxyribonuclease	-
CD796_RS07650	1590643..1590942	+	300	WP_003098543.1	hypothetical protein	-
CD796_RS07655	1590980..1591654	+	675	WP_003098542.1	hypothetical protein	-
CD796_RS07660	1591771..1592199	-	429	WP_003098541.1	hypothetical protein	Antitoxin
CD796_RS07665	1592246..1592428	-	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
CD796_RS07670	1592587..1593162	+	576	WP_157738327.1	GspH/FimT family pseudopilin	-
CD796_RS07675	1593346..1593678	+	333	WP_003098538.1	phage holin, lambda family	-
CD796_RS07680	1593675..1594292	+	618	WP_023118135.1	glycoside hydrolase family 19 protein	-
CD796_RS07685	1594310..1594741	+	432	WP_171960445.1	hypothetical protein	-
CD796_RS07690	1594833..1595252	+	420	WP_079279250.1	HNH endonuclease	-
CD796_RS07695	1595384..1595794	+	411	WP_020750362.1	P27 family phage terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1570697..1615158	44461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T78061 WP_003098540.1 NZ_CP021999:c1592428-1592246 [Pseudomonas aeruginosa]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T78061 NZ_CP021999:c1592428-1592246 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAAGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAGATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15779.98 Da
• Isoelectric Point: 4.7974

>AT78061 WP_003098541.1 NZ_CP021999:c1592199-1591771 [Pseudomonas aeruginosa]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKVHAIGEAIDAIESTLSLYVDQRREIPAASHAQPGERVIHLPAVTVA
KIALWNEMVRRDMRKADLCRLLGIAQTQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEAA

Nucleotide

• Length: 429 bp

>AT78061 NZ_CP021999:c1592199-1591771 [Pseudomonas aeruginosa]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTTGCCGTTTTCTGCAGAGACTTGCCGGAGCT
GAACAGCTATGGCGACGACAAGGTCCACGCAATCGGCGAGGCAATCGACGCCATCGAGTCGACCCTCTCGCTGTACGTTG
ATCAGCGCCGAGAAATACCCGCAGCCAGTCACGCACAACCAGGCGAGCGCGTTATCCATCTGCCGGCAGTTACCGTTGCG
AAGATCGCGCTCTGGAACGAAATGGTCCGTCGAGATATGCGAAAGGCTGACCTCTGCCGGCTTCTCGGGATCGCACAGAC
CCAGGGCGACAGGCTCGTCGACTTCCTCCACAACACTAAGATGGAGGCTATGGAGAACGCTCTATCCGCCCTCGGACTCC
GCCTCTCGGTGAATATCGAGGCAGCATGA